酶联免疫测定法。I. 胰岛素-β-D-半乳糖苷酶偶联物的新型合成方法及其在胰岛素测定中的应用。
Enzyme-linked immunoassay. I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay.
作者信息
Kato K, Hamaguchi Y, Fukui H, Ishikawa E
出版信息
J Biochem. 1975 Jul;78(1):235-7.
PMID:1104600
Abstract
Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase conjugate could be used for immunoassay of insulin.
摘要
将猪胰岛素进行巯基琥珀酰化处理，然后使用N，N'-邻苯二甲酰亚胺马来酰亚胺将其与来自大肠杆菌的β-D-半乳糖苷酶[EC 3.2.1.23]偶联。测试了该偶联物和胰岛素与抗胰岛素抗体的竞争性结合。结果表明，胰岛素-β-D-半乳糖苷酶偶联物的形成可用于胰岛素的免疫测定。
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