• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

酶联免疫测定法。I. 胰岛素-β-D-半乳糖苷酶偶联物的新型合成方法及其在胰岛素测定中的应用。

Enzyme-linked immunoassay. I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay.

作者信息

Kato K, Hamaguchi Y, Fukui H, Ishikawa E

出版信息

J Biochem. 1975 Jul;78(1):235-7.

PMID:1104600
Abstract

Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase conjugate could be used for immunoassay of insulin.

摘要

将猪胰岛素进行巯基琥珀酰化处理，然后使用N，N'-邻苯二甲酰亚胺马来酰亚胺将其与来自大肠杆菌的β-D-半乳糖苷酶[EC 3.2.1.23]偶联。测试了该偶联物和胰岛素与抗胰岛素抗体的竞争性结合。结果表明，胰岛素-β-D-半乳糖苷酶偶联物的形成可用于胰岛素的免疫测定。

相似文献

1
Enzyme-linked immunoassay. I. Novel method for synthesis of the insulin-beta-D-galactosidase conjugate and its applicability for insulin assay.酶联免疫测定法。I. 胰岛素-β-D-半乳糖苷酶偶联物的新型合成方法及其在胰岛素测定中的应用。
J Biochem. 1975 Jul;78(1):235-7.
2
Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.酶联免疫测定：兔免疫球蛋白G的Fab'片段与大肠杆菌β-D-半乳糖苷酶的偶联及其在免疫测定中的应用。
J Immunol. 1976 Jun;116(6):1554-60.
3
Enzyme coupled immunoassay of insulin using a novel coupling reagent.使用新型偶联试剂的胰岛素酶联免疫分析
J Biochem. 1976 Jan;79(1):233-6. doi: 10.1093/oxfordjournals.jbchem.a131053.
4
Improved procedure for the conjugation of rabbit IgG and Fab' antibodies with beta-D-galactosidase from Escherichia coli using N,N'-o-phenylenedimaleimide.使用N，N'-邻苯二甲酰亚胺马来酰亚胺改进兔IgG和Fab'抗体与大肠杆菌β-D-半乳糖苷酶偶联的方法。
J Biochem. 1979 May;85(5):1289-300.
5
[Preparation of an insulin conjugate with beta-galactosidase from E. coli for immunoenzyme assay].[用大肠杆菌β-半乳糖苷酶制备胰岛素缀合物用于免疫酶测定]
Bioorg Khim. 1986 Mar;12(3):327-31.
6
Enzyme-linked immunoassay. Conjugation of rabbit anti-(human immunoglobulin G) antibody with beta-D-galactosidase from Escherichia coli and its use for human immunoglobulin G assay.
Eur J Biochem. 1976 Feb 16;62(2):285-92. doi: 10.1111/j.1432-1033.1976.tb10159.x.
7
Enzyme-linked immunoassay. II. A simple method for synthesis of the rabbit antibody-beta-D-galactosidase complex and its general applicability.酶联免疫测定法。II. 兔抗体-β-D-半乳糖苷酶复合物的一种简单合成方法及其普遍适用性。
J Biochem. 1975 Aug;78(2):423-5. doi: 10.1093/oxfordjournals.jbchem.a130923.
8
Steric hindrance enzyme immunoassay (SHEIA); a novel method in enzyme immunoassay.
Res Commun Chem Pathol Pharmacol. 1979 Oct;26(1):187-96.
9
A simple method for the conjugation of affinity-purified Fab' to horseradish peroxidase and beta-D-galactosidase from Escherichia coli.一种将亲和纯化的Fab'与来自大肠杆菌的辣根过氧化物酶和β-D-半乳糖苷酶偶联的简单方法。
J Immunoassay. 1985;6(1-2):111-23. doi: 10.1080/01971528508063024.
10
Conjugation of recombinant reverse transcriptase of HIV-1 to beta-D-galactosidase from Escherichia coli for ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) of anti-HIV-1 IgG.将HIV-1重组逆转录酶与来自大肠杆菌的β-D-半乳糖苷酶偶联，用于抗HIV-1 IgG的超灵敏酶免疫测定（免疫复合物转移酶免疫测定）。
J Immunol Methods. 1994 Jun 24;172(2):179-87. doi: 10.1016/0022-1759(94)90105-8.

引用本文的文献

1
Characterization of alpha-fetoprotein levels in three dolphin species: development of sensitive immunoassays for analysis of the pregnancy-associated variations.三种海豚物种中甲胎蛋白水平的特征：用于分析妊娠相关变化的灵敏免疫测定法的开发
J Reprod Dev. 2013;59(4):368-77. doi: 10.1262/jrd.2012-061. Epub 2013 May 8.
2
Enzyme-linked sandwich immunoassay for insulin using laser fluorimetric detection.采用激光荧光检测的胰岛素酶联夹心免疫测定法。
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1901-5. doi: 10.1073/pnas.78.3.1901.
3
Enzyme-linked immunoassays for the detection of microbial antigens and their antibodies.
用于检测微生物抗原及其抗体的酶联免疫测定法。
Adv Appl Microbiol. 1986;31:271-92. doi: 10.1016/s0065-2164(08)70445-3.
4
Immunoconjugates: applications in targeted drug delivery for cancer therapy.免疫偶联物：在癌症治疗的靶向药物递送中的应用。
Pharm Res. 1987 Jun;4(3):181-8. doi: 10.1023/a:1016443725167.
5
Enzyme immunoassay of buprenorphine.丁丙诺啡的酶免疫测定法。
Naunyn Schmiedebergs Arch Pharmacol. 1988 Aug;338(2):202-6. doi: 10.1007/BF00174871.
6
Selective laser-induced inactivation of proteins (SLIP) by labelling with chromophores.通过用发色团标记实现蛋白质的选择性激光诱导失活（SLIP）。
Med Biol Eng Comput. 1992 Jul;30(4):CE17-20. doi: 10.1007/BF02446173.