Pinto J A, Brewer D B
J Pathol. 1975 Jul;116(3):149-64. doi: 10.1002/path.1711160304.
Morphometric observations were made on rats injected subcutaneously daily with puromycin aminonucleoside after two, four, six and eight daily injections. As a result it was possible to correlate the morphological alterations of the various glomerular components with the biochemical manifestations of the nephrotic syndrome particularly the onset of proteinuria. There was already significant loss of foot processes of the glomerular epithelial cells by the 2nd day, that is before the onset of proteinuria, suggesting that the loss of foot processes is a consequence of some metabolic impairment produced by the puromycin aminonucleoside which also subsequently causes the proteinuria. Swelling of the glomerular epithelial cells became significant by the 6th and 8th day of injection after the development of proteinuria, suggesting that it is a consequence of the increased amount of protein in the glomerular filtrate. Two independent morphometric methods suggest a decrease in the amount of glomerular basement membrane. Unfortunately because of difficulties in the determination of mean glomerular volumes it is not possible to be absolutely certain that this is a true finding, however it is what would be expected if the synthesis of basement membrane was depressed by the puromycin aminonucleoside.
对每日皮下注射嘌呤霉素氨基核苷的大鼠，在分别注射2次、4次、6次和8次后进行形态测量观察。结果发现，各种肾小球成分的形态学改变与肾病综合征的生化表现，尤其是蛋白尿的出现之间存在关联。在第2天，即蛋白尿出现之前，肾小球上皮细胞的足突就已经有明显丢失，这表明足突丢失是嘌呤霉素氨基核苷产生的某种代谢损伤的结果，而这种损伤随后也会导致蛋白尿。在蛋白尿出现后的注射第6天和第8天，肾小球上皮细胞肿胀变得明显，这表明它是肾小球滤过液中蛋白质含量增加的结果。两种独立的形态测量方法提示肾小球基底膜数量减少。遗憾的是，由于确定平均肾小球体积存在困难，无法绝对确定这是一个真实的发现，然而，如果嘌呤霉素氨基核苷抑制了基底膜的合成，这正是所预期的结果。
