Kaplan B S, Renaud L, Drummond K N
Lab Invest. 1976 Feb;34(2):174-8.
The purpose of these experiments was to determine whether reported changes in substrate metabolism by isolated glomeruli from rats with aminonucleoside nephrosis could be explained by the glomerular changes associated with proteinuria or, alternatively, whether these metabolic changes and proteinuria were synchronous but causally unrelated events. Aminonucleoside of puromycin produced proteinuria within 7 days when injected intraperitoneally or subcutaneously. However, when aminonucleoside of puromycin as well as adenine were given, the onset of proteinuria was delayed until after day 7. A significant reduction in U-14C-glucose oxidation to CO2 was found at day 7 by glomeruli from rats given aminonucleoside of puromycin intraperitoneally but no significant changes were found with aminonucleoside of puromycin given subcutaneously on days 7 and 9 and aminonucleoside of puromycin + adenine given subcutaneously on days 7 and 9. Rats given daunomycin or adriamycin had developed proteinuria by day 14. U-14C-glucose oxidation to CO2 was significantly reduced on day 14 in glomeruli from rats given daunomycin but no significant changes were found on day 21 with daunomycin, or on days 14 and 21 with adriamycin. There was a reduction in pyruvic-acid carbon metabolism but not in glutamine-carbon oxidation 14 days after treatment with daunomycin. These results suggest that the observed changes in glomerular metabolism occur independently of, albeit synchronous with, the development of proteinuria. A causal relationship between these metabolic alterations and proteinuria therefore may be unlikely.
这些实验的目的是确定，对于嘌呤霉素氨基核苷肾病大鼠分离出的肾小球所报告的底物代谢变化，是可以用与蛋白尿相关的肾小球变化来解释，还是相反，这些代谢变化和蛋白尿是同步但无因果关系的事件。嘌呤霉素氨基核苷经腹腔内或皮下注射后，7天内会产生蛋白尿。然而，当同时给予嘌呤霉素氨基核苷和腺嘌呤时，蛋白尿的出现会延迟到第7天之后。腹腔内注射嘌呤霉素氨基核苷的大鼠，其肾小球在第7天时U-14C-葡萄糖氧化为二氧化碳的量显著减少，但皮下注射嘌呤霉素氨基核苷在第7天和第9天，以及皮下注射嘌呤霉素氨基核苷+腺嘌呤在第7天和第9天时，均未发现显著变化。给予柔红霉素或阿霉素的大鼠在第14天时出现蛋白尿。给予柔红霉素的大鼠，其肾小球在第14天时U-14C-葡萄糖氧化为二氧化碳的量显著减少，但在第21天给予柔红霉素时，以及在第14天和第21天给予阿霉素时，均未发现显著变化。柔红霉素治疗14天后，丙酮酸碳代谢减少，但谷氨酰胺碳氧化未减少。这些结果表明，观察到的肾小球代谢变化虽然与蛋白尿的发生同步，但却是独立发生的。因此，这些代谢改变与蛋白尿之间可能不存在因果关系。
