伯氏疟原虫体内非保护性抗乳酸脱氢酶抗体对红细胞的一些体外侵袭抑制作用。

Some in vitro invasion inhibition of red cells by in vivo nonprotective anti-LDH antibodies of Plasmodium berghei.

作者信息

Kumar S

机构信息

Department of Veterinary Microbiology, Punjab Agricultural University, Ludhiana, India.

出版信息

J Vet Med B Infect Dis Vet Public Health. 2000 Sep;47(7):481-6. doi: 10.1046/j.1439-0450.2000.00316.x.

DOI:10.1046/j.1439-0450.2000.00316.x

PMID:11048428

Abstract

In Plasmodium berghei, sephadex G-200 purified lactate dehydrogenase (LDH) fraction immunized mice did not exhibit protection when challenged with 1 x 10(6) P. berghei-parasitized erythrocytes. However, LDH immunized mice seroconverted and showed an antibody titre of 1:2048 by indirect haemagglutination (IHA) and 1:160 by indirect fluorescent antibody (IFA) assays. Fluorescence was distributed evenly on P. berghei-parasitized red cells showing no specific location of parasite LDH. Anti-LDH antibodies supplemented in 19 h in vitro culture of P. berghei exhibited 9.2% invasion inhibition into the fresh red cells.

摘要

在伯氏疟原虫中，用葡聚糖G - 200纯化的乳酸脱氢酶（LDH）组分免疫的小鼠，在用1×10⁶个感染伯氏疟原虫的红细胞进行攻击时未表现出保护作用。然而，LDH免疫的小鼠发生了血清转化，通过间接血凝试验（IHA）显示抗体滴度为1:2048，通过间接荧光抗体（IFA）试验显示为1:160。荧光均匀分布在感染伯氏疟原虫的红细胞上，未显示寄生虫LDH的特定位置。在伯氏疟原虫的体外19小时培养中补充抗LDH抗体，对新鲜红细胞的侵袭抑制率为9.2%。