Sina B J, do Rosario V E, Woollett G, Sakhuja K, Hollingdale M R
Malaria Department, Biomedical Research Institute, Rockville, Maryland 20852.
Exp Parasitol. 1993 Sep;77(2):129-35. doi: 10.1006/expr.1993.1069.
Irradiated sporozoites are generally thought to elicit protective immune responses that are parasite stage and species specific. But immunization with Plasmodium falciparum sporozoites delivered by the bite of infected mosquitoes protects an average of 60% mice from Plasmodium berghei sporozoite infection. Protection appears to be specific as P. falciparum sporozoite-immunized mice protected against P. berghei remain susceptible to Plasmodium yoelii sporozoite infection. Passively transferred immunoglobulin from P. falciparum sporozoite-immunized mouse serum protected naive mice against challenge with P. berghei sporozoites, indicating that cross-protection is mediated, at least in part, by anti-sporozoite antibody. Antibody-mediated protection may not be due to cross-reaction between P. falciparum and P. berghei CS proteins because mice immunized with the recombinant P. falciparum CS protein repeat vaccine candidate, R32tet32, remain susceptible to P. berghei sporozoite infection.
一般认为，经辐照的子孢子会引发具有寄生虫阶段和物种特异性的保护性免疫反应。但是，通过感染蚊子叮咬传递的恶性疟原虫子孢子免疫，平均可使60%的小鼠免受伯氏疟原虫子孢子感染。这种保护似乎具有特异性，因为经恶性疟原虫子孢子免疫且对伯氏疟原虫有抵抗力的小鼠，对约氏疟原虫子孢子感染仍易感。从经恶性疟原虫子孢子免疫的小鼠血清中被动转移的免疫球蛋白，可保护未免疫的小鼠免受伯氏疟原虫子孢子攻击，这表明交叉保护至少部分是由抗子孢子抗体介导的。抗体介导的保护可能并非由于恶性疟原虫和伯氏疟原虫环子孢子蛋白（CS）之间的交叉反应，因为用重组恶性疟原虫CS蛋白重复疫苗候选物R32tet32免疫的小鼠，对伯氏疟原虫子孢子感染仍易感。
