用于微自显影术的制备均匀核乳剂层的新技术。

New techniques for the preparation of uniform layers of nuclear emulsions for use in micro-autoradiography.

作者信息

Sanderson J

出版信息

J Microsc. 1975 Jul;104(2):179-85. doi: 10.1111/j.1365-2818.1975.tb04015.x.

DOI:10.1111/j.1365-2818.1975.tb04015.x

Abstract

A procedure is described whereby liquid emulsion can be used to coat glass coverslips to produce films of emulsion suitable for quantitative evaluation of grain density in micro-autoradiographs. Any thickness of emulsion layer can be produced from 3 mum upwards, depending on the volume and dilution of the emulsion dispensed. The uniformity and reproducibility of the films have been tested by observing light transmission of undeveloped preparations using a microscope photometer.

摘要

本文描述了一种方法，通过该方法可以使用液体乳剂涂覆玻璃盖玻片，以制备适合于对显微放射自显影片中的颗粒密度进行定量评估的乳剂膜。根据所分配乳剂的体积和稀释度，可以制备出从3微米以上的任何厚度的乳剂层。通过使用显微镜光度计观察未显影制剂的透光率，测试了膜的均匀性和可重复性。