Boomsma F, van Dijk J, Bhaggoe U M, Bouhuizen A M, van den Meiracker A H
Cardiovascular Research Center, Division of Internal Medicine I, Erasmus University Medical Center Rotterdam, The Netherlands.
Comp Biochem Physiol C Toxicol Pharmacol. 2000 May;126(1):69-78. doi: 10.1016/s0742-8413(00)00101-8.
Semicarbazide-sensitive amine oxidase (SSAO) (E.C. 1.4.3.6) is a group of enzymes with as yet poorly understood function which is widely present in nature. The variation in methodology for determination of activity, differences in substrates used and in nomenclature have made it difficult to compare SSAO in different species and tissues. Since SSAO is implicated in the pathophysiology of diabetes mellitus and congestive heart failure, our aim was to analyse the importance and abundance of SSAO in human plasma and tissues compared to other mammals. In plasma of ten different mammals, Vmax values were found to vary more than 10,000-fold, while KM differed much less; in human plasma SSAO activity is relatively low. In some species more than one SSAO entity was present in plasma. SSAO activity was ubiquitous in tissues of human, rat and pig, but varied considerably, both between species and between tissues. In human tissues, SSAO activity is higher than in tissues from rat and pig. Relative to monoamine oxidase-B there is also wide variation in SSAO, with much higher relative activities in human than in rat and pig tissues. We conclude that in plasma, SSAO activity is highest in ruminants, while in tissues, SSAO activity is more prominently present in human than in rat and pig.
氨基脲敏感性胺氧化酶（SSAO）（E.C. 1.4.3.6）是一类功能尚未完全明确的酶，广泛存在于自然界。活性测定方法的差异、所用底物的不同以及命名的差异使得比较不同物种和组织中的SSAO变得困难。由于SSAO与糖尿病和充血性心力衰竭的病理生理学有关，我们的目的是分析与其他哺乳动物相比，SSAO在人血浆和组织中的重要性及丰度。在十种不同哺乳动物的血浆中，发现Vmax值变化超过10000倍，而KM的差异则小得多；人血浆中的SSAO活性相对较低。在某些物种的血浆中存在不止一种SSAO实体。SSAO活性在人、大鼠和猪的组织中普遍存在，但在物种之间和组织之间差异很大。在人体组织中，SSAO活性高于大鼠和猪的组织。相对于单胺氧化酶-B，SSAO也存在很大差异，在人体中的相对活性远高于大鼠和猪的组织。我们得出结论，在血浆中，反刍动物的SSAO活性最高，而在组织中，人比大鼠和猪更显著地存在SSAO活性。
