Tahara S, Kurotani R, Sanno N, Takumi I, Yoshimura S, Osamura R Y, Teramoto A
Department of Neurosurgery, Nippon Medical School, Tokyo, Japan.
Mod Pathol. 2000 Oct;13(10):1097-108. doi: 10.1038/modpathol.3880204.
We investigated the localization of pituitary homeo box 1 (Ptx1) protein in five human non-neoplastic pituitaries and 73 of all types of pituitary adenomas using immunohistochemistry, and the expression of Ptx1 messenger RNA (mRNA) in 18 representative pituitary adenomas using the reverse transcriptase polymerase chain reaction (RT-PCR) technique. By immunohistochemical analysis, Ptx1 protein was extensively detected in the nuclei of normal human pituitary cells. Ptx1 was detected in 10/14 (71.4%) of growth hormone (GH)-secreting adenomas, 12/12 (100%) of prolactin (PRL)-secreting adenomas, 18/20 (90%) of adrenocorticotropic hormone (ACTH)-secreting adenomas, 6/7 (85.7%) of thyroid-stimulating hormone (TSH)-secreting adenomas, and 17/20 (85%) of clinically non-functioning adenomas, including 9/10 (90%) of gonadotropin-subunit-positive adenomas. Thus, there was no relationship between Ptx1 expression and a particular type of pituitary adenomas. By RT-PCR analysis, Ptx1 mRNA was expressed in all 18 cases of pituitary adenomas, including two cases negative for Ptx1 protein by immunohistochemistry. These results suggested that Ptx1 may be an universal transcription factor in both neoplastic and non-neoplastic conditions in human pituitaries. The synergistic action with other transcription factors may be speculated to determine the specific production of the anterior pituitary hormones.
我们采用免疫组织化学方法研究了垂体同源盒1（Ptx1）蛋白在5例人非肿瘤性垂体及73例所有类型垂体腺瘤中的定位，并运用逆转录聚合酶链反应（RT-PCR）技术检测了18例代表性垂体腺瘤中Ptx1信使核糖核酸（mRNA）的表达。通过免疫组织化学分析，在正常人垂体细胞的细胞核中广泛检测到Ptx1蛋白。在分泌生长激素（GH）的腺瘤中，10/14（71.4%）检测到Ptx1；在分泌催乳素（PRL）的腺瘤中，12/12（100%）检测到Ptx1；在分泌促肾上腺皮质激素（ACTH）的腺瘤中，18/20（90%）检测到Ptx1；在分泌促甲状腺激素（TSH）的腺瘤中，6/7（85.7%）检测到Ptx1；在临床无功能腺瘤中，17/20（85%）检测到Ptx1，其中包括9/10（90%）促性腺激素亚单位阳性的腺瘤。因此，Ptx1表达与特定类型的垂体腺瘤之间无相关性。通过RT-PCR分析，在所有18例垂体腺瘤中均检测到Ptx1 mRNA表达，其中包括2例免疫组织化学检测Ptx1蛋白为阴性的病例。这些结果提示，Ptx1可能是人类垂体肿瘤性和非肿瘤性状态下的一种普遍转录因子。推测其与其他转录因子的协同作用可能决定垂体前叶激素的特异性产生。
