Worthington T, Lambert P A, Elliott T S
Department of Clinical Microbiology, University Hospital Birmingham NHS Trust, Queen Elizabeth, Edgbaston, Birmingham, B15 2TH, UK.
J Hosp Infect. 2000 Oct;46(2):130-4. doi: 10.1053/jhin.2000.0810.
Macrorestriction fragment profile analysis by pulsed field gel electrophoresis was used to type strains of coagulase-negative staphylococci (CNS) isolated from 30 patients with catheter-related sepsis at the University Hospital Birmingham NHS Trust, UK. Twenty-three infections were hospital-acquired. A total of 56 CNS were isolated from the patients and identified by API as Staphylococcus epidermidis (54), Staphylococcus lugdunensis (1) and Staphylococcus hominis (1). The micro-organisms were further characterized by antibiograms and restriction digestion using SmaI. Analysis of the macrorestriction fragment profiles demonstrated that the isolates from 24 patients were distinct, whereas a common genotype of S. epidermidis was isolated from the blood cultures of six patients, all of whom had acquired this infection in hospital. Three of these patients were located in a haematology ward, two on an intensive care unit and one on a dialysis unit. The data from this current study suggests that specific strains of S. epidermidis may be an important cause of nosocomial catheter-related sepsis resulting from cross-infection, and that this association would not be detected by conventional typing methods including biotyping and antibiograms.
采用脉冲场凝胶电泳进行宏观限制性片段谱分析，对从英国伯明翰大学医院国民保健服务信托基金的30例导管相关败血症患者中分离出的凝固酶阴性葡萄球菌（CNS）菌株进行分型。23例感染为医院获得性感染。共从患者中分离出56株CNS，并通过API鉴定为表皮葡萄球菌（54株）、路邓葡萄球菌（1株）和人葡萄球菌（1株）。通过抗菌谱和使用SmaI进行限制性消化对微生物进行进一步表征。宏观限制性片段谱分析表明，来自24例患者的分离株各不相同，而从6例患者的血培养物中分离出了一种常见的表皮葡萄球菌基因型，所有这些患者均在医院获得该感染。其中3例患者在血液科病房，2例在重症监护病房，1例在透析病房。当前这项研究的数据表明，特定的表皮葡萄球菌菌株可能是交叉感染导致医院导管相关败血症的重要原因，并且这种关联无法通过包括生物分型和抗菌谱在内的传统分型方法检测到。
