Karłowski W M, Strózycki P M, Legocki A B
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.
Acta Biochim Pol. 2000;47(2):371-83.
The LlPRP2 gene coding for a proline-rich protein shows a high level of similarity to, as well as significant differences from the family of ENOD2 nodule-specific genes. Several sequence motifs with putative regulatory function were identified in the 5' and 3' noncoding regions of the LlPRP2 gene. Northern blot analysis revealed that the expression of the LlPRP2 gene begins 9 days after inoculation of yellow lupin roots with Bradyrhizobium sp. (Lupinus); the expression is restricted to symbiotic nodules and is not detected in other tissues or organs. Detailed hybridization analysis showed that, when expression is activated, the LlPRP2 transcript is modified so as to produce at least three bands and a continuous distribution of decay intermediates. The modification of the LlPRP2 transcript probably involves degradation from the 5'- and/or 3'-ends of the RNA molecules. Southern blot analysis indicates that only one gene is present in the yellow lupin genome. The presence of genes homologous to the LlPRP2 gene was confirmed for three cultivars of yellow lupin and for Lupinus angustifolius. However, LlPRP2 homologues were not detected in Lupinus albus cv. Bac, indicating that this plant may lack the ENOD2 sequence.
编码富含脯氨酸蛋白的LlPRP2基因与ENOD2结节特异性基因家族具有高度相似性，但也存在显著差异。在LlPRP2基因的5'和3'非编码区鉴定出了几个具有假定调控功能的序列基序。Northern印迹分析表明，在用慢生根瘤菌（Lupinus）接种黄羽扇豆根9天后，LlPRP2基因开始表达；该表达仅限于共生结节，在其他组织或器官中未检测到。详细的杂交分析表明，当表达被激活时，LlPRP2转录本会发生修饰，从而产生至少三条带以及衰变中间体的连续分布。LlPRP2转录本的修饰可能涉及RNA分子5'端和/或3'端的降解。Southern印迹分析表明，黄羽扇豆基因组中仅存在一个基因。已证实黄羽扇豆的三个品种和窄叶羽扇豆中存在与LlPRP2基因同源的基因。然而，在白羽扇豆品种Bac中未检测到LlPRP2同源物，这表明该植物可能缺乏ENOD2序列。
