Nuc K, Nuc P, Słomski R
August Cieszkowski University of Agriculture, Department of Biochemistry and Biotechnology, Poznań, Poland.
Mol Plant Microbe Interact. 2001 Dec;14(12):1384-94. doi: 10.1094/MPMI.2001.14.12.1384.
Cyclophilin (CyP) is one of the enzymes that act as peptidylprolyl cis-trans isomerases (EC 5.2.1.8). The cDNA and an intronless gene coding for cytosolic CyP have been isolated from yellow lupine. The deduced amino acid sequence of the characterized open reading frame shows approximately 80% homology with cytosolic CyP from other organisms. Southern blots of genomic DNA indicate that there is a small family of genes for CyP-related genes in the yellow lupine genome. RNA blot analyses demonstrate that CyP genes are expressed in all plant organs. The amount of CyP transcripts is dramatically increased in root nodules. In situ hybridization experiments indicate that CyP transcripts are localized mainly in meristematic tissues, with the highest level observed in the nodule meristem zone. The promoter of the sequenced gene contains 5' AAAGAT 3' and AT-rich motifs that are characteristic for some nodulin promoters.
亲环蛋白（CyP）是一种作为肽基脯氨酰顺反异构酶起作用的酶（EC 5.2.1.8）。已从黄羽扇豆中分离出编码胞质CyP的cDNA和一个无内含子基因。所鉴定的开放阅读框推导的氨基酸序列与其他生物的胞质CyP显示出约80%的同源性。基因组DNA的Southern印迹表明，黄羽扇豆基因组中存在一个与CyP相关基因的小基因家族。RNA印迹分析表明，CyP基因在所有植物器官中均有表达。根瘤中CyP转录本的量显著增加。原位杂交实验表明，CyP转录本主要定位于分生组织，在根瘤分生组织区观察到最高水平。测序基因的启动子含有5' AAAGAT 3'和富含AT的基序，这些是一些根瘤蛋白启动子的特征。
