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从人淋巴细胞中检测损伤识别蛋白。

Detection of damage-recognition proteins from human lymphocytes.

作者信息

Lanuszewska J, Cudak A, Rzeszowska-Wolny J, Widłak P

机构信息

Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.

出版信息

Acta Biochim Pol. 2000;47(2):443-50.

PMID:11051209
Abstract

Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.

摘要

对从健康供体获取的人淋巴细胞中识别并结合受损DNA的蛋白质（DDB蛋白）进行了分析。使用电泳迁移率变动分析，检测到核提取物蛋白与受损DNA之间形成了几种复合物：一种对经N - 乙酰氧基 - N - 乙酰氨基芴损伤的DNA具有特异性的复合物，另一种对紫外线照射的DNA具有特异性的复合物，以及两种对顺式二氯二氨铂损伤的DNA具有特异性的复合物。所有检测到的复合物在电泳迁移率上有所不同，并且可能包含不同的蛋白质。在淋巴细胞的蛋白质提取物中也检测到了对游离DNA末端具有特异性的复合物。

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Detection of damage-recognition proteins from human lymphocytes.从人淋巴细胞中检测损伤识别蛋白。
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Characterization of a novel protein that specifically binds to DNA modified by N-acetoxy-acetylaminofluorene and cis-diamminedichloroplatinum.一种特异性结合由N-乙酰氧基-乙酰氨基芴和顺式二氯二氨铂修饰的DNA的新型蛋白质的表征。
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Damaged DNA-binding proteins: recognition of N-acetoxy-acetylaminofluorene-induced DNA adducts.受损的DNA结合蛋白：对N-乙酰氧基-乙酰氨基芴诱导的DNA加合物的识别
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Detection, purification and characterization of a protein that binds the (6-4) photoproduct-containing DNA in HeLa cells.在HeLa细胞中检测、纯化和鉴定一种与含（6-4）光产物的DNA结合的蛋白质。
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Mitochondrial transcription factor A is the major protein in rodent hepatocytes that recognizes DNA lesions induced by N-acetoxy-acetylaminofluorene.线粒体转录因子A是啮齿动物肝细胞中识别由N-乙酰氧基-乙酰氨基芴诱导的DNA损伤的主要蛋白质。
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