从人淋巴细胞中检测损伤识别蛋白。
Detection of damage-recognition proteins from human lymphocytes.
作者信息
Lanuszewska J, Cudak A, Rzeszowska-Wolny J, Widłak P
机构信息
Department of Experimental and Clinical Radiobiology, Center of Oncology, Gliwice, Poland.
出版信息
Acta Biochim Pol. 2000;47(2):443-50.
Proteins recognizing and binding to damaged DNA (DDB-proteins) were analyzed in human lymphocytes obtained from healthy donors. Using an electrophoretic mobility shift assay several complexes between nuclear extract proteins and damaged DNA were detected: a complex specific for DNA damaged by N-acetoxy-N-acetylaminofluorene, another complex specific for UV-irradiated DNA, and two complexes specific for DNA damaged by cis-dichlorodiammine platinum. All the detected complexes differed in electrophoretic mobility and possibly contained different proteins. Complexes specific for free DNA ends were also detected in protein extracts from lymphocytes.
对从健康供体获取的人淋巴细胞中识别并结合受损DNA的蛋白质（DDB蛋白）进行了分析。使用电泳迁移率变动分析，检测到核提取物蛋白与受损DNA之间形成了几种复合物：一种对经N - 乙酰氧基 - N - 乙酰氨基芴损伤的DNA具有特异性的复合物，另一种对紫外线照射的DNA具有特异性的复合物，以及两种对顺式二氯二氨铂损伤的DNA具有特异性的复合物。所有检测到的复合物在电泳迁移率上有所不同，并且可能包含不同的蛋白质。在淋巴细胞的蛋白质提取物中也检测到了对游离DNA末端具有特异性的复合物。
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