Tagashira M, Yamamoto K, Fujio K, Nagano T, Okamoto R, Ibuki N, Yabushita K, Matsumura S, Okano N, Tsuji T
First Department of Internal Medicine, Okayama University Medical School, Japan.
J Clin Immunol. 2000 Sep;20(5):347-53. doi: 10.1023/a:1006668013276.
Cytotoxic T lymphocytes (CTLs) play an important role in the pathogenesis of viral hepatitis. We studied the expression of mRNAs of perforin and Fas ligand (Fas-L) in biopsy specimens from chronic hepatitis B (CHB) (15 cases) and hepatitis C (CHC) patients (13 cases). Both perforin and Fas-L mRNAs were detected in all cases of both CHB and CHC. No messages were detected in the control livers from two cases of fatty liver, a case of Gilbert's syndrome, and a case of Dubin-Johnson syndrome. Semiquantitative analysis revealed a positive correlation between the intensity of perforin and Fas-L mRNAs in both CHB and CHC. In CHB, the intensity of both perforin and Fas-L mRNAs showed a positive correlation with the histological activity and serum alanine aminotransferase level, while the correlation was not apparent in CHC. These results suggest that both perforin and Fas/Fas-L systems are involved in the pathogenesis of liver cell injury of CHB and CHC.
细胞毒性T淋巴细胞（CTLs）在病毒性肝炎发病机制中起重要作用。我们研究了慢性乙型肝炎（CHB，15例）和丙型肝炎（CHC，13例）患者活检标本中穿孔素和Fas配体（Fas-L）mRNA的表达。CHB和CHC所有病例均检测到穿孔素和Fas-L mRNA。在2例脂肪肝、1例吉尔伯特综合征和1例杜宾-约翰逊综合征患者的对照肝脏中未检测到相关信息。半定量分析显示，CHB和CHC中穿孔素和Fas-L mRNA强度呈正相关。在CHB中，穿孔素和Fas-L mRNA强度均与组织学活性及血清丙氨酸氨基转移酶水平呈正相关，而在CHC中这种相关性不明显。这些结果提示，穿孔素和Fas/Fas-L系统均参与CHB和CHC肝细胞损伤的发病机制。
