病毒性肝炎患者肝脏中穿孔素和Fas配体mRNA的表达

Expression of perforin and Fas ligand mRNA in the liver of viral hepatitis.

作者信息

Tagashira M, Yamamoto K, Fujio K, Nagano T, Okamoto R, Ibuki N, Yabushita K, Matsumura S, Okano N, Tsuji T

机构信息

First Department of Internal Medicine, Okayama University Medical School, Japan.

出版信息

J Clin Immunol. 2000 Sep;20(5):347-53. doi: 10.1023/a:1006668013276.

DOI:10.1023/a:1006668013276

PMID:11051276

Abstract

Cytotoxic T lymphocytes (CTLs) play an important role in the pathogenesis of viral hepatitis. We studied the expression of mRNAs of perforin and Fas ligand (Fas-L) in biopsy specimens from chronic hepatitis B (CHB) (15 cases) and hepatitis C (CHC) patients (13 cases). Both perforin and Fas-L mRNAs were detected in all cases of both CHB and CHC. No messages were detected in the control livers from two cases of fatty liver, a case of Gilbert's syndrome, and a case of Dubin-Johnson syndrome. Semiquantitative analysis revealed a positive correlation between the intensity of perforin and Fas-L mRNAs in both CHB and CHC. In CHB, the intensity of both perforin and Fas-L mRNAs showed a positive correlation with the histological activity and serum alanine aminotransferase level, while the correlation was not apparent in CHC. These results suggest that both perforin and Fas/Fas-L systems are involved in the pathogenesis of liver cell injury of CHB and CHC.

摘要

细胞毒性T淋巴细胞（CTLs）在病毒性肝炎发病机制中起重要作用。我们研究了慢性乙型肝炎（CHB，15例）和丙型肝炎（CHC，13例）患者活检标本中穿孔素和Fas配体（Fas-L）mRNA的表达。CHB和CHC所有病例均检测到穿孔素和Fas-L mRNA。在2例脂肪肝、1例吉尔伯特综合征和1例杜宾-约翰逊综合征患者的对照肝脏中未检测到相关信息。半定量分析显示，CHB和CHC中穿孔素和Fas-L mRNA强度呈正相关。在CHB中，穿孔素和Fas-L mRNA强度均与组织学活性及血清丙氨酸氨基转移酶水平呈正相关，而在CHC中这种相关性不明显。这些结果提示，穿孔素和Fas/Fas-L系统均参与CHB和CHC肝细胞损伤的发病机制。