Qian Q, Wu M, Qu Z
Tumor Immunology and Gene Therapy Center, Eastern Institute of Hepatobiliary Surgery, Shanghai 200433.
Zhonghua Bing Li Xue Za Zhi. 1998 Apr;27(2):99-101.
To investigate perforin and Fas-ligand (Fas-L) expression of tumor infiltrating lymphocytes (TIL) in human hepatocellular carcinoma (HCC).
Expression of perforin and Fas-L of TIL was studied in 20 HCC cases using in situ hybridization and immunohistochemistry.
Expression of perforin and Fas-L gene were detected in TIL in 80% of the cases studied. Among them, one patient (no. 14) in whom expression of perforin and Fas-L were noticed in the majority of the TIL, had no recurrence of HCC for one and one half (1.5) year after tumor resection. It indicates that presence of large number of activated T cells might be beneficial for the tumor therapy. In the remaining cases, only 10% of TIL were obtained able to express perforin and Fas-L indicating that only a few TIL were activated and cytotoxic to HCC.
Even there were a multitude of T cells infiltrating in HCC, only few of them were immunoactived and to be cytotoxic to HCC. It seems important to adopt measures in order to promote further proliferation of these activated T cells either in vitro or in vivo.
研究人肝细胞癌（HCC）中肿瘤浸润淋巴细胞（TIL）的穿孔素和Fas配体（Fas-L）表达。
采用原位杂交和免疫组化方法，研究20例HCC病例中TIL的穿孔素和Fas-L表达。
在所研究的病例中，80%的TIL检测到穿孔素和Fas-L基因表达。其中，1例患者（第14号）的大多数TIL中均观察到穿孔素和Fas-L表达，肿瘤切除后1年半未出现HCC复发。这表明大量活化T细胞的存在可能对肿瘤治疗有益。在其余病例中，仅10%的TIL能够表达穿孔素和Fas-L，表明仅有少数TIL被激活并对HCC具有细胞毒性。
即使HCC中有大量T细胞浸润，但其中仅有少数具有免疫活性并对HCC具有细胞毒性。采取措施促进这些活化T细胞在体外或体内进一步增殖似乎很重要。
