Trapp B D, Dwyer B, Bernsohn J
Neurobiology. 1975 Oct;5(5):235-48.
Astrocytes and neuronal and oligodendroglial perikarya isolated by the method of Norton and Poduslo (1970) were examined by transmission and scanning electron microscopy and inverted phase contrast microscopy. The viability of the cells, as determined by the eosin exclusion method, was also determined. The three cell fractions showed only slight cross-contamination, but the astrocyte fraction contained significant amount of small debris. The ultrastructural appearance of the cells indicated that much of the in situ properties were retained, with bundles of fibrils preserved in astrocytes with well-defined plasma membranes. Oligodendroglial perikarya were found to be the best preserved of the cell types. The viability studies indicated that about 90% of the cells excluded eosin. Scanning electron microscopy revealed the neuronal cell surface to be rough and studded with knob-like bodies. Oligodendrocytes tended to aggregate and demonstrated a much smoother surface than the neurons.
通过诺顿和波杜斯洛（1970年）的方法分离出的星形胶质细胞、神经元细胞以及少突胶质细胞的胞体，采用透射电子显微镜、扫描电子显微镜以及倒置相差显微镜进行了检查。还通过伊红排斥法测定了细胞的活力。这三种细胞组分仅显示出轻微的交叉污染，但星形胶质细胞组分含有大量小碎片。细胞的超微结构外观表明，细胞保留了许多原位特性，星形胶质细胞中保存有纤维束，其质膜清晰可辨。发现少突胶质细胞的胞体是这几种细胞类型中保存最好的。活力研究表明，约90%的细胞排斥伊红。扫描电子显微镜显示神经元细胞表面粗糙，布满瘤状小体。少突胶质细胞倾向于聚集，其表面比神经元表面光滑得多。
