Lee S E, Lees E M, Campbell B C
Plant Protection Research Unit, Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 800 Buchanan Street, Albany, California 94710, USA.
J Agric Food Chem. 2000 Oct;48(10):4991-6. doi: 10.1021/jf991258f.
Esterases from a fenitrothion-resistant strain (VOSF) of the saw-toothed grain beetle, Oryzaephilus surinamensis (L.), are presumed to play a role in conferring resistance to malathion, fenitrothion, and chlorpyrifos-methyl. Colorimetric assays showed a significant positive correlation between increased resistance to fenitrothion in strains of O. surinamensis examined and elevated esterase hydrolytic activity to substrates of p-nitrophenyl acetate, alpha-naphthyl acetate, and beta-naphthyl acetate. Esterase zymograms showed different banding patterns between VOSF and an insecticide-susceptible strain, VOS48. A major esterase in the VOSF strain, not detected in VOS48, was purified and characterized by chromatographic and electrophoretic techniques. On the basis of SDS-polyacrylamide gel eletrophoresis, the molecular mass of the purified esterase from VOSF was 130 kDa and consisted of two 65 kDa subunits. Additional properties of this enzyme are discussed.
据推测，锯谷盗（Oryzaephilus surinamensis (L.)）的杀螟硫磷抗性品系（VOSF）中的酯酶在赋予对马拉硫磷、杀螟硫磷和甲基毒死蜱的抗性方面发挥作用。比色法测定表明，在所检测的锯谷盗品系中，对杀螟硫磷抗性的增加与对乙酸对硝基苯酯、乙酸α-萘酯和乙酸β-萘酯底物的酯酶水解活性升高之间存在显著正相关。酯酶同工酶谱显示VOSF和杀虫剂敏感品系VOS48之间存在不同的条带模式。通过色谱和电泳技术纯化并表征了VOSF品系中一种在VOS48中未检测到的主要酯酶。基于SDS-聚丙烯酰胺凝胶电泳，来自VOSF的纯化酯酶的分子量为130 kDa，由两个65 kDa的亚基组成。讨论了该酶的其他特性。
