Simar L J
Pathol Biol (Paris). 1975 Jun;23(6):444-7.
Previous investigations have shown that, during a primary reaction against a single injection of heterologous red blood cells in the Mouse, immunoblasts migrate from the lymph node germinal centers to the medullary cords through the paracortical area. In that study, we combined ultrastructural examination with stereological methods in order to analyze the differentiation of the immunocompetent cells during this migration. The data permit one to characterize two important events in this differentiation. Between the 12th and 24th hour after the antigen injection, when the germinal centers are being dispersed, the cytoplasmic volume of the cells increases 2.5 fold. The estimated number of ribosomes per cell increases from 350,000 to 1 X 10(6) and the volumetric and surface densities of endoplasmic reticulum increase by a factor of 10. Four days later, 120 hours after the antigen administration, the immunoblasts, now located in the medullary cords, show a spectacular increase in the volume and surface density of endoplasmic reticulum, and in the surface density of Golgi membranes. This change results in the transformation of immunoblasts into young plasma cells. Between these two developments, there is a three day period during which the immunoblasts undergo little change.
先前的研究表明，在小鼠针对单次注射异种红细胞的初次反应过程中，免疫母细胞从淋巴结生发中心通过副皮质区迁移至髓索。在该研究中，我们将超微结构检查与体视学方法相结合，以分析免疫活性细胞在这一迁移过程中的分化情况。这些数据使人们能够描述这种分化过程中的两个重要事件。在抗原注射后12至24小时之间，当生发中心开始分散时，细胞的细胞质体积增加2.5倍。每个细胞中核糖体的估计数量从350,000增加到1×10⁶，内质网的体积密度和表面密度增加了10倍。四天后，即抗原给药120小时后，此时位于髓索中的免疫母细胞在内质网的体积和表面密度以及高尔基体膜的表面密度方面均出现显著增加。这种变化导致免疫母细胞转变为年轻的浆细胞。在这两个发育阶段之间，有一个为期三天的时间段，在此期间免疫母细胞几乎没有变化。
