Andoh A, Takaya H, Araki Y, Tsujikawa T, Fujiyama Y, Bamba T
Department of Internal Medicine, Shiga University of Medical Science, Seta-Tukinowa, Otsu 520-2192, Japan.
J Nutr. 2000 Nov;130(11):2636-40. doi: 10.1093/jn/130.11.2636.
The primary therapeutic effects of enteral nutrition in patients with Crohn's disease have been reported previously. Although the quantity and type of fat in enteral nutrition are considered to be important, it is unclear how fat modulates mucosal inflammatory responses in the intestine. In the present study, we evaluated the effects of medium-chain and long-chain fatty acids (MCFA and LCFA) on interleukin (IL)-8 secretion in a fetal intestinal epithelial cell line, intestine-407 cells. IL-8 expression was evaluated at the protein and mRNA levels. The activation of nuclear factor-kappaB was assessed with an electrophoretic gel mobility shift assay. The addition of oleic acid (LCFA) micelles, but not octanoic acid (MCFA) micelles, weakly but significantly enhanced basal IL-8 secretion in the intestine-407 cells. The addition of MCFA (5 mmol/L) induced a 40% increase in IL-1beta-induced IL-8 secretion and a 35% increase in tumor necrosis factor (TNF)-alpha-induced IL-8 secretion, respectively. The addition of LCFA (5 mmol/L) induced a 140% increase in IL-1beta-induced IL-8 secretion and a 110% increase in TNF-alpha-induced IL-8 secretion, respectively. These responses were also observed at the mRNA levels. The electrophoretic gel mobility shift assay indicated that both MCFA and LCFA enhanced IL-1beta- and TNF-alpha-induced nuclear factor-kappaB activation. We demonstrated the proinflammatory activities of MCFA and especially LCFA. It is likely that medium-chain triglycerides may be more suitable than long-chain triglycerides as an energy source in enteral diets in the treatment of patients with Crohn's disease.
肠内营养对克罗恩病患者的主要治疗作用此前已有报道。尽管肠内营养中脂肪的数量和类型被认为很重要，但脂肪如何调节肠道黏膜炎症反应尚不清楚。在本研究中，我们评估了中链脂肪酸和长链脂肪酸（MCFA和LCFA）对胎儿肠上皮细胞系Intestine-407细胞白细胞介素（IL）-8分泌的影响。在蛋白质和mRNA水平评估IL-8表达。用电泳凝胶迁移率变动分析评估核因子-κB的激活。添加油酸（LCFA）微团而非辛酸（MCFA）微团可轻微但显著增强Intestine-407细胞的基础IL-8分泌。添加MCFA（5 mmol/L）分别使IL-1β诱导的IL-8分泌增加40%，肿瘤坏死因子（TNF）-α诱导的IL-8分泌增加35%。添加LCFA（5 mmol/L）分别使IL-1β诱导的IL-8分泌增加140%，TNF-α诱导的IL-8分泌增加110%。在mRNA水平也观察到了这些反应。电泳凝胶迁移率变动分析表明，MCFA和LCFA均增强了IL-1β和TNF-α诱导的核因子-κB激活。我们证明了MCFA尤其是LCFA的促炎活性。在克罗恩病患者的肠内饮食中，中链甘油三酯可能比长链甘油三酯更适合作为能量来源。
