Chondrogianni N, Petropoulos I, Franceschi C, Friguet B, Gonos E S
National Hellenic Research Foundation, Laboratory of Molecular and Cellular Aging, Institute of Biological Research and Biotechnology, 48 Vas. Constantinou Avenue, 11635, Athens, Greece.
Exp Gerontol. 2000 Sep;35(6-7):721-8. doi: 10.1016/s0531-5565(00)00137-6.
Healthy centenarians represent the best example of successful ageing. Various studies have shown that centenarians have escaped the major age-associated diseases, they have several well-conserved immune parameters and at least one gene allele has been identified and linked with their increased longevity. During ageing there is an accumulation of oxidised proteins, a phenomenon that has been related to an impaired function of the 20S proteasome in aged cells. We have, therefore, analysed the expression and the proteolytic activity of the proteasome in centenarian cells. Four fibroblast cultures derived from healthy centenarians were studied and compared with cultures derived from adult donors of different ages. Analysis of several proteasome subunits RNA expression levels, determination of one peptidase activity and identification of oxidised proteins in these samples revealed that centenarian cultures have a functional proteasome. In addition, it was found that the centenarian cultures exhibit characteristics similar to the younger rather than the older control donors derived cultures in all three assays. These data indicate that centenarian cells may be different from elderly donors cells, thus opening up new dimensions for the identification and characterisation of factors that are linked with longevity.
健康的百岁老人是成功衰老的最佳典范。多项研究表明，百岁老人避开了与年龄相关的主要疾病，他们有几个保存良好的免疫参数，并且至少有一个基因等位基因已被确定与他们的长寿增加有关。在衰老过程中，氧化蛋白质会积累，这一现象与老年细胞中20S蛋白酶体功能受损有关。因此，我们分析了百岁老人细胞中蛋白酶体的表达和蛋白水解活性。研究了来自健康百岁老人的四种成纤维细胞培养物，并与来自不同年龄成年供体的培养物进行了比较。对这些样品中几种蛋白酶体亚基RNA表达水平的分析、一种肽酶活性的测定以及氧化蛋白质的鉴定表明，百岁老人的培养物具有功能性蛋白酶体。此外，发现在所有这三项检测中，百岁老人的培养物表现出与年轻而非年长对照供体来源的培养物相似的特征。这些数据表明，百岁老人的细胞可能与老年供体细胞不同，从而为识别和表征与长寿相关的因素开辟了新的维度。
