酸通过大鼠胃黏液层通道的转运

Acid transport through channels in the mucous layer of rat stomach.

作者信息

Johansson M, Synnerstad I, Holm L

机构信息

Department of Physiology, Uppsala University, Uppsala, Sweden.

出版信息

Gastroenterology. 2000 Nov;119(5):1297-304. doi: 10.1053/gast.2000.19455.

DOI:10.1053/gast.2000.19455

PMID:11054387

Abstract

BACKGROUND & AIMS: We have reported previously that secreted acid moves through the mucous layer in restricted areas above the gastric crypts. The aim of this study was to visualize and study the dynamics of this event.

METHODS

Anesthetized rats prepared for intravital microscopy of the gastric mucosa were divided in the following groups with respect to acid secretion: spontaneous; stimulated (pentagastrin, 40 microg. kg(-1). h(-1)); transiently inhibited (omeprazole, 400 micromol. kg(-1) for 7 days); and totally inhibited (omeprazole, 3 x 200 micromol. kg(-1) for 7 days). The mucus was stained with Congo red (blue, pH < 3; red, pH > 5.2), and photographs were taken through a stereomicroscope.

RESULTS

During acid secretion, blue-colored crypt openings with attached thread-like (5-7 microm wide) structures (designated channels) were seen passing from the crypt openings through the mucus to the lumen. Red-colored channels and crypt openings were observed when acid secretion was transiently inhibited. Red-colored crypt openings but no channels were found after total inhibition of acid secretion for a week.

CONCLUSIONS

The results suggest that secreted acid is transported through channels within the mucus. These channels are probably created by the high intraglandular pressure pushing acid and glandular mucus into the gel.

摘要

背景与目的

我们之前曾报道，分泌的酸在胃小凹上方的受限区域穿过黏液层。本研究的目的是观察并研究这一过程的动态变化。

方法

为进行胃黏膜活体显微镜检查而麻醉的大鼠，根据酸分泌情况分为以下几组：自发分泌组；刺激分泌组（五肽胃泌素，40微克·千克⁻¹·小时⁻¹）；短暂抑制分泌组（奥美拉唑，400微摩尔·千克⁻¹，持续7天）；完全抑制分泌组（奥美拉唑，3×200微摩尔·千克⁻¹，持续7天）。用刚果红对黏液进行染色（pH<3时呈蓝色；pH>5.2时呈红色），并通过体视显微镜拍照。