Davis-Taber R, Choi W, Feng J, Hoogenboom L, McNally T, Kroeger P, Shieh C C, Simmer R, Brioni J D, Sullivan J P, Gopalakrishnan M, Scott V E
Neurological and Urological Diseases Research, Dept. 47C, APgA, Abbott Laboratories, 100 Abbott Park Road, Abbott Park, IL 60064, USA.
Gene. 2000 Oct 3;256(1-2):261-70. doi: 10.1016/s0378-1119(00)00338-3.
The distribution of human sulfonylurea receptor-2 (SUR2)-containing K(ATP) channels was investigated using reverse transcriptase-polymerase chain reaction (RT-PCR). mRNA for SUR2B was detected in a variety of tissues including brain, skeletal, cardiac and smooth muscle, whereas SUR2A message was restricted to cardiac and skeletal muscle. An additional splice variant of SUR2 that lacked exon 17 was also identified by RT-PCR in tissues expressing both SUR2A and SUR2B or SUR2B alone. Quantification of RNA for SUR2 exon 17+ and SUR2 exon 17- splice variants using real-time Taqman PCR indicated differential levels of expression in brain, kidney, skeletal muscle, heart and small intestine. Interestingly, the SUR2 exon 17+ variant is the major species expressed in all tissues examined in this study. Each of the SUR2 splice variants transiently expressed with the inward rectifier Kir 6.2 formed functional K(ATP) channels in HEK 293 cells as assessed either by changes in DiBAC(4)(3) fluorescence responses or glyburide-sensitive whole cell currents. Collectively, our findings demonstrate that various SUR2 splice variants have distinct expression patterns and can form functional K(ATP) channels.
运用逆转录聚合酶链反应（RT-PCR）对含人磺脲类受体2（SUR2）的K（ATP）通道的分布进行了研究。在包括脑、骨骼肌、心肌和平滑肌在内的多种组织中检测到了SUR2B的mRNA，而SUR2A的信息则局限于心肌和骨骼肌。通过RT-PCR在单独表达SUR2A和SUR2B或仅表达SUR2B的组织中还鉴定出了一种缺少外显子17的SUR2额外剪接变体。使用实时Taqman PCR对SUR2外显子17 +和SUR2外显子17 -剪接变体的RNA进行定量分析，结果表明在脑、肾、骨骼肌、心脏和小肠中表达水平存在差异。有趣的是，SUR2外显子17 +变体是本研究中所有检测组织中表达的主要类型。通过DiBAC(4)(3)荧光反应变化或格列本脲敏感的全细胞电流评估，在HEK 293细胞中，每个与内向整流器Kir 6.2瞬时共表达的SUR2剪接变体均形成了功能性K（ATP）通道。总的来说，我们的研究结果表明，各种SUR2剪接变体具有不同的表达模式，并且能够形成功能性K（ATP）通道。
