Shimura N, Musya A, Hashimoto T, Kojima S, Kubodera A, Sasaki T
Faculty of Pharmaceutical Sciences, Science University of Tokyo, Tokyo, Japan.
Nucl Med Biol. 2000 Aug;27(6):577-80. doi: 10.1016/s0969-8051(00)00131-1.
To investigate whether [(99m)Tc]-hexamethyl propyleneamine oxime ([(99m)Tc]-HMPAO) is applicable for evaluating glutathione (GSH) localization in tumor, the difference of distribution between [(99m)Tc]-d,l- and meso-HMPAO was studied using a mouse tumor model. Biodistribution of [(99m)Tc]-d,l- or meso-HMPAO was studied in GSH-depleted and control Ehrlich tumor-bearing mice. GSH levels in tumors in GSH-depleted and control mice were measured in another set of mice. The uptake of [(99m)Tc]-d,l-HMPAO in tumor was significantly decreased by the diethyl maleate (DEM) treatment. On the other hand, the DEM treatment increased the accumulation of [(99m)Tc]-meso-HMPAO in tumor. Meanwhile, the content of GSH was lowest in tumor among the tissues tested and decreased in a manner similar to other tissues on preloading of DEM. [(99m)Tc]-d,l-HMPAO may be useful for estimating the GSH status in a certain tumor and thereby contribute to the diagnosis of anticancer therapy.
为研究[（99m）Tc] - 六甲基丙烯胺肟（[（99m）Tc] - HMPAO）是否适用于评估肿瘤中谷胱甘肽（GSH）的定位，利用小鼠肿瘤模型研究了[（99m）Tc] - d，l - HMPAO和内消旋 - HMPAO之间的分布差异。在GSH耗竭的和对照的荷艾氏瘤小鼠中研究了[（99m）Tc] - d，l - HMPAO或内消旋 - HMPAO的生物分布。在另一组小鼠中测量了GSH耗竭的和对照小鼠肿瘤中的GSH水平。马来酸二乙酯（DEM）处理显著降低了[（99m）Tc] - d，l - HMPAO在肿瘤中的摄取。另一方面，DEM处理增加了[（99m）Tc] - 内消旋 - HMPAO在肿瘤中的蓄积。同时，在所测试的组织中，肿瘤中GSH的含量最低，并且在预先加载DEM时以与其他组织相似的方式降低。[（99m）Tc] - d，l - HMPAO可能有助于评估特定肿瘤中的GSH状态，从而有助于抗癌治疗的诊断。
