Kishi H, Bao J, Kohama K
Department of Pharmacology and First Department of Internal Medicine, Gunma University School of Medicine, Gunma 371-8511, Japan.
J Biochem. 2000 Nov;128(5):719-22. doi: 10.1093/oxfordjournals.jbchem.a022806.
The chemotactic migration toward platelet-derived growth factor-BB of SM3, a cell line established from rabbit aorta smooth muscle, was examined by the Boyden chamber method. Myosin light-chain (MLC) kinase inhibitors ML-9 and wortmannin, and the Rho kinase inhibitor Y-27632 effectively reduced the migration. However, neither membrane ruffling nor the phosphorylation of MLC was inhibited concomitantly. The reduction is discussed with reference to a novel property of MLC kinase, which stimulates myosin ATPase activity without phosphorylating MLC [Ye et al. (1999) Proc. Natl. Acad. Sci. USA 96, 6666-6671].
采用博伊登小室法检测了从兔主动脉平滑肌建立的细胞系SM3对血小板衍生生长因子-BB的趋化迁移。肌球蛋白轻链（MLC）激酶抑制剂ML-9和渥曼青霉素以及Rho激酶抑制剂Y-27632可有效降低迁移。然而，膜 ruffling和MLC的磷酸化均未同时受到抑制。结合MLC激酶的一种新特性对这种降低进行了讨论，该特性可刺激肌球蛋白ATP酶活性而不使MLC磷酸化[Ye等人（1999年），《美国国家科学院院刊》96，6666 - 6671]。
