嗜热栖热球菌吡咯烷酮羧基肽酶的克隆、表达及特性分析
Cloning, expression, and characterization of pyrrolidone carboxyl peptidase from the archaeon Thermococcus litoralis.
作者信息
Singleton M R, Taylor S J, Parrat J S, Littlechild J A
机构信息
Department of Chemistry, University of Exeter, UK.
出版信息
Extremophiles. 2000 Oct;4(5):297-303. doi: 10.1007/s007920070017.
PMID:11057915
Abstract
The gene encoding pyrrolidone carboxyl peptidase (Pcp) has been cloned from the hyperthermophilic archaeon Thermococcus litoralis. The recombinant enzyme has been expressed in Escherichia coli, purified, and characterized. The T. litoralis Pcp demonstrates strong sequence homology to previously characterized bacterial Pcps. Some investigations have been carried out on enzyme substrate specificity and stability.
摘要
编码吡咯烷酮羧肽酶（Pcp）的基因已从嗜热古菌嗜热栖热球菌中克隆出来。该重组酶已在大肠杆菌中表达、纯化并进行了特性鉴定。嗜热栖热球菌Pcp与先前鉴定的细菌Pcp具有很强的序列同源性。已对该酶的底物特异性和稳定性进行了一些研究。
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