抗体在海藻酸钠-壳聚糖微珠上的固定化。

Immobilization of antibodies on alginate-chitosan beads.

作者信息

Albarghouthi M, Fara D A, Saleem M, El-Thaher T, Matalka K, Badwan A

机构信息

Chemical Engineering Department, University of Jordan, 11942, Amman, Jordan.

出版信息

Int J Pharm. 2000 Sep 25;206(1-2):23-34. doi: 10.1016/s0378-5173(00)00470-1.

DOI:10.1016/s0378-5173(00)00470-1

PMID:11058807

Abstract

An anti-hapten IgG was covalently immobilized on glutaraldehyde-activated alginate-chitosan gel beads. The antibody immobilization efficiency was influenced by glutaraldehyde-bead reaction time, IgG concentration and pH. In addition, immobilization conditions such as glutaraldehyde and antibody concentrations influenced antibody hapten binding affinity. The immobilized IgG on the beads was stable and no reduction in the percent binding to hapten was noticed following 25 days of storage. It was concluded that antibodies could be successfully immobilized on alginate-chitosan gel beads. Such a system can be applied for the development of immunoaffinity purification and immunoassays.

摘要

一种抗半抗原IgG被共价固定在戊二醛活化的海藻酸钠-壳聚糖凝胶珠上。抗体固定效率受戊二醛与珠子的反应时间、IgG浓度和pH值的影响。此外，诸如戊二醛和抗体浓度等固定条件会影响抗体与半抗原的结合亲和力。固定在珠子上的IgG是稳定的，在储存25天后，与半抗原的结合百分比没有降低。得出的结论是，抗体可以成功地固定在海藻酸钠-壳聚糖凝胶珠上。这样的系统可应用于免疫亲和纯化和免疫测定的开发。