Chen P, Zhai W, Zhang Y, Zhang J, Gu Y
Shanghai Medical University, Shanghai 200032, China.
Zhonghua Gan Zang Bing Za Zhi. 2000 Oct;8(5):276-8.
To study the effect of hypoxia on the expression and activity of matrix metalloproteinase-2 (MMP-2) in the hepatic stellate cell (HSC).
The expression of MMP-2 and tissue inhibitor of metalloproteinase-2 (TIMP-2) in cultured rat HSC was detected by immunocytochemistry (ICC). The relative amount of MMP-2 and TIMP-2 in the culture supernatant was assayed by ELISA and the activity of MMP-2 in supernatant was examined by zymography.
The expression of MMP-2 was enhanced in hypoxia group as compared with that in control one (t' 3.4685, P<0.05 ), while the expression of TIMP-2 was decreased (t'=2.5673, P<0.05). The activity of MMP-2 was lower in hypoxia group than in control one (t'=4.270, P<0.01). Comparing the varied duration of hypoxia, the relative amount of MMP-2 protein was the highest in 6 h group (6h vs 12h, t=4. 0945, P<0.001; 6h vs 24h, t=2.4652, P<0.025), with a contrary curve in TIMP-2.
Hypoxia promotes the expression of MMP-2 and inhibits the expression of TIMP-2 in HSC. It is more notable at early stage of hypoxia.
研究缺氧对肝星状细胞（HSC）中基质金属蛋白酶-2（MMP-2）表达及活性的影响。
采用免疫细胞化学法（ICC）检测培养的大鼠HSC中MMP-2和金属蛋白酶组织抑制剂-2（TIMP-2）的表达。用酶联免疫吸附测定法（ELISA）检测培养上清液中MMP-2和TIMP-2的相对含量，并用酶谱分析法检测上清液中MMP-2的活性。
与对照组相比，缺氧组MMP-2的表达增强（t' = 3.4685，P < 0.05），而TIMP-2的表达降低（t' = 2.5673，P < 0.05）。缺氧组MMP-2的活性低于对照组（t' = 4.270，P < 0.01）。比较不同缺氧时间，MMP-2蛋白相对含量在6小时组最高（6小时 vs 12小时，t = 4.0945，P < 0.001；6小时 vs 24小时，t = 2.4652，P < 0.025），TIMP-2呈相反趋势。
缺氧促进HSC中MMP-2的表达并抑制TIMP-2的表达。在缺氧早期这种作用更显著。
