[甘氨脱氧胆酸诱导的大鼠肝细胞凋亡]

[Rat hepatocellular apoptosis induced by glycodeoxycholate].

作者信息

Huang W, Kang G, Yang M, Tu Z, Chen H, Tang Z, Jiang H, Yan Y

机构信息

Dept. of Clinical Laboratory, Sichuan Provincial People's Hospital, Chengdu 610027, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2000 Oct;8(5):305-6.

PMID:11058960

Abstract

OBJECTIVE

To explore the relationship between glycodeoxycholate (GDC) and rat hepatocellular apoptosis.

METHODS

GDC was used to treat rat hepatocytes cultured in vitro and the apoptotic cells were observed and analyzed with light microscope, transmission electron microscope, TUNEL in situ hybridization, DNA agarose gel electrophoresis and flow cytometry.

RESULTS

When rat hepatocytes were incubated with GDC (final concentration of 100 micromol/L) for 2h, apoptotic hepatocytes were observed with light and transmission electron microscope, and TUNEL in situ hybridization. When the hepatocytes were incubated with GDC (final concentration of 50, 100, 150 micromol/L, respectively) for 2h, or with GDC (final concentration of 100 micromol/L) for 2, 4, 6, 8h, respectively, agarose gel electrophoresis of the hepatocytes DNA demonstrated the typical ladder patterns.

CONCLUSION

GDC with final concentration of 50, 100, 150 micromol/L could induce rat hepatocellular apoptosis.

摘要

目的

探讨甘氨脱氧胆酸（GDC）与大鼠肝细胞凋亡之间的关系。

方法

用GDC处理体外培养的大鼠肝细胞，采用光学显微镜、透射电子显微镜、TUNEL原位杂交、DNA琼脂糖凝胶电泳及流式细胞术对凋亡细胞进行观察和分析。

结果

当大鼠肝细胞与终浓度为100μmol/L的GDC孵育2小时后，通过光学显微镜、透射电子显微镜及TUNEL原位杂交观察到凋亡的肝细胞。当肝细胞分别与终浓度为50、100、150μmol/L的GDC孵育2小时，或与终浓度为100μmol/L的GDC分别孵育2、4、6、8小时后，肝细胞DNA的琼脂糖凝胶电泳呈现典型的梯状条带。