Wu M B, Xia L M, Cen P L
Institute of Bioengineering, Zhejiang University, Hangzhou.
Sheng Wu Gong Cheng Xue Bao. 2000 May;16(3):368-72.
The cells of Trichoderma reesei were immobilized on a roll of porous polyurethane foam sheet and packed in a bubble column bioreactor for simultaneous production of chitosanase and degradation of chitosan in situ. The average degree of polymerization could be regulated by reaction time. Under the repeated-batch process with 2% soluble chitosan at pH4.8, 28 degrees C, the activity of chitosanase for each batch was above 0.15 IU/mL, the average yield of reducing sugar as D-glucosamine reached 73%. The novel immobilized bioreactor system run stably and effectively in the successive 10 batches lasting 30 days without notable change in the activity and productivity.
里氏木霉细胞被固定在一卷多孔聚氨酯泡沫板上，并装填于鼓泡塔生物反应器中，用于原位同步生产壳聚糖酶和降解壳聚糖。聚合度平均值可通过反应时间来调节。在pH4.8、28℃条件下，以2%的可溶性壳聚糖进行重复分批操作时，每批壳聚糖酶的活性均高于0.15 IU/mL，以D-葡萄糖胺计的还原糖平均产率达到73%。这种新型固定化生物反应器系统在连续10批、持续30天的运行过程中稳定且高效，酶活性和生产力没有明显变化。
