Kim Y S, Jin S H, Lee Y H, Park J D, Kim S I
Korea Ginseng and Tobacco Research Institute, Youseong-Gu, Taejon.
Arch Pharm Res. 2000 Oct;23(5):518-24. doi: 10.1007/BF02976583.
We examined the modulation of protein kinase C (PKC) subtypes during apoptosis induced by ginsenoside Rh2 (G-Rh2) in human neuroblastoma SK-N-BE(2) and rat glioma C6Bu-1 cells. Apoptosis induced by G-Rh2 in both cell lines was confirmed, as indicated by DNA fragmentation and in situ strand breaks, and characteristic morphological changes. During apoptosis induced by G-Rh2 in SK-N-BE(2) cells, PKC subtypes alpha, beta and gamma were progressively increased with prolonged treatment, whereas PKC delta increased transiently at 3 and 6 h and PKC epsilon was gradually down-regulated after 6 h following the treatment. On the other hand, PKC subtype zeta markedly increased at 24 h when maximal apoptosis was achieved. In C6Bu-1 cells, no significant changes in PKC subtypes alpha, gamma, delta, epsilon and zeta were observed during apoptosis induced by G-Rh2. These results suggest the evidence for a possible role of PKC subtype in apoptosis induced by G-Rh2 in SK-N-BE(2) cells but not in C6Bu-1 cells, and raise the possibility that G-Rh2 may induce apoptosis via different pathways interacting with or without PKC in different cell types.
我们研究了人参皂苷Rh2（G-Rh2）诱导人神经母细胞瘤SK-N-BE(2)细胞和大鼠胶质瘤C6Bu-1细胞凋亡过程中蛋白激酶C（PKC）亚型的调节情况。DNA片段化和原位链断裂以及特征性形态变化表明，G-Rh2在两种细胞系中均诱导了细胞凋亡。在G-Rh2诱导SK-N-BE(2)细胞凋亡的过程中，随着处理时间延长，PKC亚型α、β和γ逐渐增加，而PKCδ在处理后3小时和6小时短暂增加，PKCε在处理6小时后逐渐下调。另一方面，当达到最大凋亡时，PKC亚型ζ在24小时显著增加。在C6Bu-1细胞中，G-Rh2诱导凋亡过程中未观察到PKC亚型α、γ、δ、ε和ζ的显著变化。这些结果表明，PKC亚型在G-Rh2诱导SK-N-BE(2)细胞凋亡中可能发挥作用，但在C6Bu-1细胞中并非如此，这也增加了G-Rh2可能通过与不同细胞类型中PKC相互作用或不相互作用的不同途径诱导细胞凋亡的可能性。
