Biancifiori F, Garrido F, Nielsen K, Moscati L, Durán M, Gall D
Istituto Zooprofilattico Sperimentale Umbria e Marche, Perugia, Italy.
New Microbiol. 2000 Oct;23(4):399-406.
In this study a cELISA for the diagnosis of brucellosis due to B. melitensis in sheep and goats was evaluated and its capability of discriminating vaccinated from infected animals was assessed. Information is provided indicating that the cELISA has a diagnostic sensitivity (99.4%) and specificity (98.9%) in sheep and goats comparable to that of many standard indirect ELISA methods. In addition, the test proved able to distinguish between vaccinated and infected animals with an accuracy of up to 90% and results reproducibility of 93%. It was concluded that the cELISA could be useful for differentiation of Rev.1 vaccinated and naturally infected sheep and goats.
在本研究中，对一种用于诊断绵羊和山羊中因羊种布鲁氏菌引起的布鲁氏菌病的竞争酶联免疫吸附测定（cELISA）进行了评估，并评估了其区分接种疫苗动物和感染动物的能力。所提供的信息表明，该cELISA在绵羊和山羊中的诊断敏感性（99.4%）和特异性（98.9%）与许多标准间接酶联免疫吸附测定方法相当。此外，该检测方法被证明能够区分接种疫苗和感染的动物，准确率高达90%，结果重现性为93%。得出的结论是，cELISA可用于区分接种Rev.1疫苗的绵羊和山羊与自然感染的绵羊和山羊。
