Snead M L, Paine M L, Luo W, Zhu D H, Yoshida B, Lei Y P, Paine C T, Chen L S, Burstein J M, Jitpukdeebudintra S, White S N, Bringas P
The Center for Cranirofacial Molecular Biology, The University of Southern California, Los Angeles 90033, USA.
Connect Tissue Res. 1998;38(1-4):279-86; discussion 295-303. doi: 10.3109/03008209809017048.
Understanding the cellular and molecular events that regulate the formation of enamel is a major driving force in efforts to characterize critical events during amelogenesis. It is anticipated that through such an understanding, improvements in prevention, diagnosis and treatment-intervention into heritable and acquired diseases of enamel could be achieved. While knowledge of the precise role of an enamel-specific protein in directing the formation of inorganic crystallites remains refractory, progress has been made with other aspects of amelogenesis that can be brought to bear on the subject. One such area of progress has been with the identification of an ameloblast-lineage specific amelogenin gene promoter. This promoter can be used to direct the expression of enamel-specific proteins, as well as the expression of proteins foreign to amelogenesis, into the enamel extracellular matrix where their effect on biomineralization can be ascertained in a prospective manner. The resulting enamel from such animals can be examined by morphologic and biochemical modalities in order to identify the effect of the transgene protein on enamel crystallite formation and subsequent biomineralization. This manuscript outlines such a strategy with the potential for enhancing our understanding of amelogenesis.
了解调节牙釉质形成的细胞和分子事件是表征釉质形成过程中关键事件的主要驱动力。预计通过这种了解，可以在牙釉质遗传性和后天性疾病的预防、诊断和治疗干预方面取得进展。虽然牙釉质特异性蛋白在指导无机微晶形成中的确切作用仍难以确定，但在釉质形成的其他方面已取得进展，这些进展可应用于该主题。其中一个进展领域是鉴定了成釉细胞谱系特异性釉原蛋白基因启动子。该启动子可用于将牙釉质特异性蛋白以及釉质形成过程中不存在的蛋白质表达定向到牙釉质细胞外基质中，从而可以前瞻性地确定它们对生物矿化的影响。可以通过形态学和生化方法检查来自此类动物的牙釉质，以确定转基因蛋白对牙釉质微晶形成和随后生物矿化的影响。本手稿概述了这样一种策略，该策略有可能增强我们对釉质形成的理解。
