Murrough J W, Boss-Williams K A, Emery M S, Bonsall R W, Weiss J M
Department of Psychiatry and Behavioral Sciences, Emory University School of Medicine, Emory West Campus, 1256 Briarcliff Road, NE Atlanta, GA 30322, USA.
Brain Res. 2000 Nov 10;883(1):125-30. doi: 10.1016/s0006-8993(00)02850-x.
6-Hydroxydopamine (6-OHDA) lesions of brain noradrenergic neurons and terminals were made in rats to assess the importance of forebrain norepinephrine (NE) for mediating circadian patterns of spontaneous ambulatory activity that rats show in the home cage. 6-OHDA was injected intracranially into the fibers of the ascending noradrenergic dorsal and ventral bundle pathways or infused into the lateral ventricle or both. Rats living in a 12/12 h light/dark cycle exhibit a marked increase in ambulatory activity during the dark period in comparison to the light period and a 'W-shaped' pattern of activity during the 12 h of the dark phase. Results showed that near-total depletion of brain NE did not impair the capacity to generate normal patterns of spontaneous ambulatory activity that occur in the home cage. In the animals that sustained the most complete NE lesions, the amounts of activity generated at times of peak activity were exaggerated in comparison to the control animals, which is consistent with the possibility that NE in the brain exerts a moderating influence on behavior.
对大鼠脑内去甲肾上腺素能神经元和终末进行6-羟基多巴胺（6-OHDA）损伤，以评估前脑去甲肾上腺素（NE）对介导大鼠在饲养笼中表现出的自发活动昼夜节律模式的重要性。将6-OHDA颅内注射到去甲肾上腺素能背侧和腹侧束上行通路的纤维中，或注入侧脑室，或两者都进行。生活在12/12小时光照/黑暗周期中的大鼠，与光照期相比，黑暗期的自发活动显著增加，并且在黑暗期的12小时内呈现“W形”活动模式。结果表明，脑内NE几乎完全耗竭并不损害在饲养笼中产生正常自发活动模式的能力。在NE损伤最完全的动物中，与对照动物相比，在活动高峰期产生的活动量有所夸大，这与脑内NE对行为发挥调节作用的可能性一致。
