新型4,15血清型脑膜炎奈瑟菌菌株的遗传学和免疫学特征
Genetic and immunologic characterization of a novel serotype 4, 15 strain of Neisseria meningitidis.
作者信息
Bash M C, Lynn F, Concepcion N F, Tappero J W, Carlone G M, Frasch C E
机构信息
Division of Bacterial Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
出版信息
FEMS Immunol Med Microbiol. 2000 Nov;29(3):169-76. doi: 10.1111/j.1574-695X.2000.tb01519.x.
The porin proteins of Neisseria meningitidis are important components of outer membrane protein (OMP) vaccines. The class 3 porin gene, porB, of a novel serogroup B, serotype 4, 15 isolate from Chile (Ch501) was found to be VR1-4, VR2-15, VR3-15 and VR4-15 by porB variable region (VR) typing. Rabbit immunization studies using outer membrane vesicles revealed immunodominance of individual PorB (class 3) VR epitopes. The predominant anti-Ch501 PorB response was directed to the VR1 epitope. Anti-PorB VR1 mediated killing was suggested by the bactericidal activity of Ch501 anti-sera against a type 4 strain not expressing PorA or class 5 OMPs. Studies that examine the molecular epidemiology of individual porB VRs, and the immune responses to PorB epitopes, may contribute to the development of broadly protective group B meningococcal vaccines.
脑膜炎奈瑟菌的孔蛋白是外膜蛋白（OMP）疫苗的重要组成部分。通过对来自智利的新型B群、4型、15型分离株（Ch501）的3类孔蛋白基因porB进行porB可变区（VR）分型，发现其为VR1-4、VR2-15、VR3-15和VR4-15。使用外膜囊泡进行的兔免疫研究揭示了单个PorB（3类）VR表位的免疫显性。针对Ch501的主要PorB反应针对VR1表位。Ch501抗血清对不表达PorA或5类OMP的4型菌株的杀菌活性表明存在抗PorB VR1介导的杀伤作用。研究单个porB VR的分子流行病学以及对PorB表位的免疫反应，可能有助于开发具有广泛保护作用的B群脑膜炎球菌疫苗。
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