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Growth on indium-tin oxide-coated glass enhances 32P-phosphate uptake and protein labelling of adherent cells.

作者信息

Tomai E, Klein S, Firth K, Raptis L

机构信息

Queen's University, Dept of Microbiology and Immunology, Kingston, Ont, Canada.

出版信息

Prep Biochem Biotechnol. 2000 Nov;30(4):313-20. doi: 10.1080/10826060008544970.

Abstract

A method to improve the efficiency of labelling of adherent cells with radioactive 32p is described. Cells are grown on a glass surface which is coated with indium-tin oxide, a commercially available, transparent material which permits excellent cell adhesion and growth. The results show that a 2 to 3-fold increase in 32p uptake by the cells can be achieved by growing cells on this material, compared to conventional tissue culture plastic.

摘要

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