Choi Chang Kyoung, Kihm Kenneth D, English Anthony E
Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville, Tennessee 37996-2210, USA.
Opt Lett. 2007 Jun 1;32(11):1405-7. doi: 10.1364/ol.32.001405.
The use of an optically thin indium-tin-oxide (ITO) electrode is presented for an optoelectric biosensor simultaneously recording optical images and microimpedance to examine time-dependent cellular growth. The transmittance of a 100 nm thick ITO electrode layer is approximately the same as the transmittance of a clean glass substrate, whereas the industry-standard Au(47.5 nm)/Ti(2.5 nm) electrode layer drops the transmittance to less than 10% of that of the glass substrate. The simultaneous optoelectric measurements permit determining the correlation of the cell-covered area increase with the microimpedance increase, and the example results obtained for live porcine pulmonary artery endothelial cells delineate the quantitative and comprehensive nature of cellular attachment and spreading to the substrate, which has not been clearly perceived before.
本文介绍了一种光学薄铟锡氧化物（ITO）电极在光电生物传感器中的应用，该传感器可同时记录光学图像和微阻抗，以检测细胞随时间的生长情况。100nm厚的ITO电极层的透光率与干净玻璃基板的透光率大致相同，而行业标准的Au(47.5nm)/Ti(2.5nm)电极层则将透光率降至玻璃基板的10%以下。同时进行的光电测量能够确定细胞覆盖面积增加与微阻抗增加之间的相关性，从活猪肺动脉内皮细胞获得的示例结果描绘了细胞在底物上附着和铺展的定量和全面特性，这在以前尚未被清晰认识到。
