Rivas Luis A, Parro Vı Ctor, Moreno-Paz Mercedes, Mellado Rafael P
Centro Nacional de Biotecnologı́a (CSIC), Campus de la Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain1.
Microbiology (Reading). 2000 Nov;146 ( Pt 11):2929-2936. doi: 10.1099/00221287-146-11-2929.
The Bacillus subtilis 168 csn gene encodes a chitosanase. It was found that transcription of the csn gene was temporally regulated and was not subject to metabolic repression. Chitosanase synthesis was abolished in a csn mutant strain. Csn was overproduced in B. subtilis, partially purified and characterized. The deduced amino acid sequence, K(m), and optimal pH and temperature of the B. subtilis enzyme were closer to those of a chitosanase from Streptomyces sp. N174 than to those of chitosanases from other Bacillus strains.
枯草芽孢杆菌168的csn基因编码一种壳聚糖酶。研究发现，csn基因的转录受到时间调控，不受代谢阻遏。在csn突变株中，壳聚糖酶的合成被消除。在枯草芽孢杆菌中过量表达Csn，进行部分纯化并对其特性进行了研究。枯草芽孢杆菌该酶的推导氨基酸序列、米氏常数（K(m)）、最适pH和温度，与链霉菌属N174的壳聚糖酶更接近，而与其他芽孢杆菌菌株的壳聚糖酶不同。
