Fu T T, Thayer D W
Biotechnol Bioeng. 1975 Dec;17(12):1749-60. doi: 10.1002/bit.260171205.
The production of protein by a Brevibacterium sp. JM98A using mesquite wood as the substrate was compared in batch and semicontinuous cultures. A 14 liter glass fermentor with automatic pH, temperature, and foam control was used for the study. A pH range of 6.6 to 7.2 was optimum for the growth of JM98A. The batch and semicontinuous cultures were compared on the basis of viable cell counts, protein production, CMC-Ase (beta-1,4-glucanase) activity, and filter paper cellulase (beta-1,4-glucan cellobiohydrolyase) activity. Total hexose, cellulose, and reducing sugar consumption were measured. The semicontinuous process yielded 2.97 times as much protein in 72 hr as the batch cultures. Most of the biomass resulted from the utilization of soluble sugars rather than from the degradation of cellulose during the semicontinuous process.
比较了短杆菌属JM98A菌株在分批培养和半连续培养中以牧豆树木材为底物生产蛋白质的情况。使用一个具有自动pH、温度和泡沫控制功能的14升玻璃发酵罐进行该研究。pH值范围为6.6至7.2对JM98A的生长最为适宜。根据活细胞计数、蛋白质产量、CMC酶（β-1,4-葡聚糖酶）活性和滤纸纤维素酶（β-1,4-葡聚糖纤维二糖水解酶）活性对分批培养和半连续培养进行了比较。测定了总己糖、纤维素和还原糖的消耗量。半连续培养过程在72小时内产生的蛋白质是分批培养的2.97倍。在半连续培养过程中，大部分生物量来自可溶性糖的利用，而非纤维素的降解。
