胸腺基质细胞和胸腺细胞产生的转化生长因子-β对早期鸡胸腺细胞增殖的调节作用

Regulation of early chicken thymocyte proliferation by transforming growth factor-beta from thymic stromal cells and thymocytes.

作者信息

Mukamoto M, Kodama H

机构信息

Laboratory of Veterinary Immunology, Department of Veterinary Science, College of Agriculture, Osaka Prefecture University, Sakai, 599-8531, Osaka, Japan.

出版信息

Vet Immunol Immunopathol. 2000 Nov 23;77(1-2):121-32. doi: 10.1016/s0165-2427(00)00223-3.

DOI:10.1016/s0165-2427(00)00223-3

PMID:11068070

Abstract

We examined expression of TGF-betas in chicken thymic stromal cells and thymocytes and roles of TGF-betas in thymocyte development within the thymus. Thymic stromal cells expressed TGF-beta 2 and 3 genes but not TGF-beta 4 gene. Thymocytes showed expressions of TGF-beta 2, 3 and 4 genes and each TGF-beta gene was expressed more strongly in CD3- than CD3+ thymocytes. When anti-TGF-beta antibody was added with supernatants of stromal cells into thymocyte culture, only proliferative activity of CD3- thymocytes was enhanced and the cells in S and G2/M compartments of cell cycle increased. These results suggest that TGF-beta which is expressed in the thymus may regulate the ability of immature thymocytes to progress through the cell cycle and to differentiate to CD3+ thymocytes.

摘要

我们检测了转化生长因子β（TGF-β）在鸡胸腺基质细胞和胸腺细胞中的表达情况，以及TGF-β在胸腺内胸腺细胞发育过程中的作用。胸腺基质细胞表达TGF-β2和TGF-β3基因，但不表达TGF-β4基因。胸腺细胞表达TGF-β2、TGF-β3和TGF-β4基因，且每个TGF-β基因在CD3-胸腺细胞中的表达均强于CD3+胸腺细胞。当将抗TGF-β抗体与基质细胞的上清液加入胸腺细胞培养体系时，仅CD3-胸腺细胞的增殖活性增强，且细胞周期中S期和G2/M期的细胞数量增加。这些结果表明，胸腺中表达的TGF-β可能调节未成熟胸腺细胞通过细胞周期以及分化为CD3+胸腺细胞的能力。