Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Molecules. 2011 Sep 30;16(10):8343-52. doi: 10.3390/molecules16108343.
Radix Glycyrrhizae polysaccharide (GP) possesses multiple pharmacological activities. However, the effect of GP on CD4+CD25+ regulatory T (Treg) cells has not been elucidated. This study aimed to investigate the effects of GP on Treg cells and Th1/Th2 cytokines in H22 hepatocarcinoma tumor-bearing mice. The results demonstrated that GP inhibits tumor progression. In the lymph nodes of the tumor microenvironment and spleen, the proportion of Treg cells was significantly higher in the tumor-bearing mice. GP administration down-regulated the population of Treg cells (P < 0.01) and decreased lymph node Foxp3 and IL-10 mRNA expression (P < 0.01). In addition, GP treatment decreased IL-10 and TGF-β level (P < 0.01) and increased IL-2 and IL-12p70 level in serum (P < 0.01). In conclusion, GP reduced the proportion of Treg cells and Foxp3 lowered expression in Treg cells, and up-regulated Th1/Th2 cytokine ratio in serum in the tumor bearing mice, which might partially cause the inhibition of tumor growth.
甘草多糖（GP）具有多种药理活性。然而，GP 对 CD4+CD25+调节性 T（Treg）细胞的影响尚未阐明。本研究旨在探讨 GP 对 H22 肝癌荷瘤小鼠 Treg 细胞及 Th1/Th2 细胞因子的影响。结果表明，GP 抑制肿瘤进展。在肿瘤微环境的淋巴结和脾脏中，荷瘤小鼠 Treg 细胞的比例明显升高。GP 给药可下调 Treg 细胞的比例（P＜0.01），并降低淋巴结 Foxp3 和 IL-10mRNA 表达（P＜0.01）。此外，GP 治疗降低了血清中 IL-10 和 TGF-β水平（P＜0.01），并增加了血清中 IL-2 和 IL-12p70 水平（P＜0.01）。综上所述，GP 降低了荷瘤小鼠 Treg 细胞的比例和 Treg 细胞中 Foxp3 的表达，并上调了血清中 Th1/Th2 细胞因子的比值，这可能部分导致肿瘤生长的抑制。
