Frost M, Bobak J B, Gianani R, Kim N, Weinrich S, Spalding D C, Cass L G, Thompson L C, Enomoto T, Uribe-Lopez D, Shroyer K R
Department of Pathology, University of Colorado Health Sciences Center, Denver 80262, USA.
Am J Clin Pathol. 2000 Nov;114(5):726-34. doi: 10.1309/XWFE-ARMN-HG2D-AJYV.
Telomerase has been detected by telomerase repeat amplification protocol (TRAP) assay in cervical dysplasia and squamous cell carcinoma but not in most normal cervical tissues. In the present study, the cellular localization of the protein catalytic subunit of telomerase (hTERT) and the RNA component (hTR) were investigated by a sensitive immunohistochemical technique and by in situ hybridization, respectively. hTERT protein was detected in all diagnostic categories of cervical specimens. hTERT was localized predominantly to the lower suprabasal levels of normal squamous mucosa but was detected throughout virtually all levels of the lesional epithelium in low-grade squamous intraepithelial lesions (LSILs), high-grade squamous intraepithelial lesions (HSILs), and squamous cell carcinoma (SCC). Telomerase expression correlated with hTERT detection in SCC and HSIL but was not detected by TRAP assay in most samples of normal mucosa or LSIL. The distribution of hTR correlated with the localization of hTERT in HSIL and SCC but was restricted to the basal and suprabasal cell layers in normal mucosa and LSIL.
通过端粒酶重复序列扩增法（TRAP）检测发现，宫颈发育异常和鳞状细胞癌中存在端粒酶，但大多数正常宫颈组织中未检测到。在本研究中，分别采用灵敏的免疫组织化学技术和原位杂交技术，对端粒酶的蛋白质催化亚基（hTERT）和RNA组分（hTR）进行细胞定位研究。在所有诊断类型的宫颈标本中均检测到hTERT蛋白。hTERT主要定位于正常鳞状黏膜的基底上层较低水平，但在低级别鳞状上皮内病变（LSIL）、高级别鳞状上皮内病变（HSIL）和鳞状细胞癌（SCC）的几乎所有病变上皮层中均能检测到。端粒酶表达与SCC和HSIL中的hTERT检测结果相关，但在大多数正常黏膜或LSIL样本中，通过TRAP检测未发现端粒酶表达。hTR的分布与HSIL和SCC中hTERT的定位相关，但在正常黏膜和LSIL中仅限于基底细胞层和基底上层细胞层。
