Momo F, Fabris S, Stevanato R
Department of Physical Chemistry, University of Venice, Italy.
Arch Biochem Biophys. 2000 Oct 15;382(2):224-31. doi: 10.1006/abbi.2000.2014.
The effect of linear monoamines on dimyristoylphosphatidylglycerol and dimyristoylphosphatidylcholine multilamellar liposomes was studied as a function of their length and compared with the behavior of linear carboxylic acids. The role of the hydrophobic interactions was demonstrated and the free energy of the binding for each interacting carbon atom was determined. The thermotropic behavior of the liposomes was characterized by differential scanning calorimetry and it was shown that these molecules affect the temperature and the cooperativity of the gel to fluid state transition of the membrane differently. In particular, it appeared that membrane perturbation was maximum when the chain length of the amphipathic molecules ranged between 7 and 9 carbon atoms, with more pronounced effects in the case of monoamines. Molecules shorter than 3-4 carbon atoms did not produce any observable change in the transition temperature. The study was extended to linear alpha,omega-diamines to investigate the amphipathic character of long diamines and to investigate the role of bridging bonds established with neighboring phospholipids.
研究了线性单胺对二肉豆蔻酰磷脂酰甘油和二肉豆蔻酰磷脂酰胆碱多层脂质体的影响，并将其作为链长的函数进行研究，同时与线性羧酸的行为进行比较。证明了疏水相互作用的作用，并确定了每个相互作用碳原子的结合自由能。通过差示扫描量热法表征脂质体的热致行为，结果表明这些分子对膜从凝胶态到流体态转变的温度和协同性有不同影响。特别地，当两亲分子的链长在7至9个碳原子之间时，膜扰动似乎最大，单胺的影响更为明显。短于3 - 4个碳原子的分子在转变温度上未产生任何可观察到的变化。该研究扩展到线性α,ω - 二胺，以研究长链二胺的两亲特性，并研究与相邻磷脂形成的桥连键的作用。
