Costa R M, Camper N D, Riley M B
Department of Microbiology and Molecular Medicine, Clemson University, SC 29634-0377, USA.
J Environ Sci Health B. 2000 Nov;35(6):677-87. doi: 10.1080/03601230009373301.
The effect of atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine) on rhizosphere microorganisms and its fate in a containerized rhizosphere system was studied. The rhizosphere system consisted of corn grown in pot containing a defined potting mix of sand and bark with atrazine. Sterilized potting mix and a container without plants served as controls. Atrazine was extracted and analyzed via HPLC. Fluorescent pseudomonad populations increased 100-fold in the rhizposphere during a 60-day incubation period as compared to the nonvegetated control. Atrazine degradation was higher in the rhizosphere system (half-life of 7 days) compared to the nonvegetated control (half-life of greater than 45 days). The major degradation product detected in the rhizosphere system was deisopropylatrazine; other products detected included deethylatrazine, deethylhydroxyatrazine, deisopropylatrazine and hydroxyatrazine. Hydroxyatrazine was detected in the nonvegetated and sterile controls. The containerized rhizosphere system provides an experimental system to study the fate of pesticidal chemicals as well as the effects on microbial populations.
研究了莠去津（2-氯-4-乙氨基-6-异丙氨基-s-三嗪）对根际微生物的影响及其在容器化根际系统中的归宿。根际系统由种植在装有含莠去津的特定砂和树皮盆栽混合物的花盆中的玉米组成。经过灭菌的盆栽混合物和没有植物的容器作为对照。通过高效液相色谱法提取和分析莠去津。与无植被对照相比，在60天的培养期内，根际荧光假单胞菌种群增加了100倍。与无植被对照（半衰期大于45天）相比，根际系统中莠去津的降解更高（半衰期为7天）。在根际系统中检测到的主要降解产物是去异丙基莠去津；检测到的其他产物包括去乙基莠去津、去乙基羟基莠去津、去异丙基莠去津和羟基莠去津。在无植被和无菌对照中检测到了羟基莠去津。容器化根际系统提供了一个实验系统，用于研究农药化学品的归宿以及对微生物种群的影响。
