Oztaş B, Erkin E, Dural E, Isbir T
Department of Physiology, Istanbul Faculty of Medicine, Turkey.
Int J Neurosci. 2000 Nov;105(1-4):27-35. doi: 10.3109/00207450009003263.
We have examined the effect of antioxidants (vitamin E, and selenium) on the blood-brain barrier permeability during adreneline-induced acute hypertension in the female rats. The rats supplemented with nontoxic doses of sodium selenite in drinking water for three months or vitamin E was given intraperitoneally before adrenaline-induced acute hypertension. Evans-blue was used as a blood-brain barrier tracer. Mean values for Evans-blue dye were found to be 0.28 +/- 0.04 microg/g tissue in control animals and 1.0 +/- 0.2 microg tissue after adrenaline-induced acute hypertension (p < .01). Rats pretreated with selenium or vitamin E also showed macroscopic leakage of Evans-blue albumin after adrenaline injection i.e., there was no significant difference in protein extravasation between untreated and treated animals (p > .5). The mean value for Evans-blue dye was found to be 1.0 +/- 0.2 microg/g tissue in acute hypertension group, 0.9 +/- 0.2 microg/g tissue in selenium pretreated animals and 1.0 +/- 0.2 micrg/g tissue vitamin E injected animals after acute hypertension. The results show that antioxidants did not influence the blood-brain barrier breakdown during adrenaline-induced acute hypertension.
我们研究了抗氧化剂（维生素E和硒）对雌性大鼠肾上腺素诱导的急性高血压期间血脑屏障通透性的影响。在肾上腺素诱导急性高血压之前，给大鼠在饮水中补充无毒剂量的亚硒酸钠三个月，或腹腔注射维生素E。伊文思蓝用作血脑屏障示踪剂。在对照动物中，伊文思蓝染料的平均值为0.28±0.04微克/克组织，在肾上腺素诱导急性高血压后为1.0±0.2微克/克组织（p<0.01）。用硒或维生素E预处理的大鼠在注射肾上腺素后也显示出伊文思蓝白蛋白的宏观渗漏，即未处理和处理动物之间的蛋白质外渗没有显著差异（p>0.5）。急性高血压组中伊文思蓝染料的平均值为1.0±0.2微克/克组织，硒预处理动物中为0.9±0.2微克/克组织，急性高血压后注射维生素E的动物中为1.0±0.2微克/克组织。结果表明，抗氧化剂在肾上腺素诱导的急性高血压期间不影响血脑屏障的破坏。
