Tuaillon N, Capra J D
Oklahoma Medical Research Foundation, Oklahoma City 73104, USA.
Eur J Immunol. 2000 Oct;30(10):2998-3005. doi: 10.1002/1521-4141(200010)30:10<2998::AID-IMMU2998>3.0.CO;2-X.
Typically, immunoglobulin VHDJH recombination is performed in two steps with D to JH rearrangement preceding VH to DJH rearrangement. Using a human immunoglobulin heavy chain transgenic minilocus, we previously demonstrated that a non-conventional human D gene segment termed DIR2 could be recombined to a VH gene segment to form VHD rearrangements. Here, we demonstrate that VHD rearrangements involve conventional D gene segments as well. VHD rearrangements are easily detected and are diverse. Similarly to DJH rearrangements, VHD rearrangements occur by deletion and inversion. They occur approximately 1000 times less frequently than DJH rearrangements. VHD rearrangements can constitute intermediates for the formation of VHDDJH rearrangements.
通常情况下，免疫球蛋白VHDJH重排分两步进行，D基因片段与JH基因片段的重排先于VH基因片段与DJH基因片段的重排。我们之前利用一个人免疫球蛋白重链转基因微基因座证明，一个名为DIR2的非常规人D基因片段可与VH基因片段重排形成VHD重排。在此，我们证明VHD重排也涉及常规D基因片段。VHD重排易于检测且具有多样性。与DJH重排类似，VHD重排通过缺失和倒位发生。其发生频率比DJH重排低约1000倍。VHD重排可构成VHDDJH重排形成的中间体。
