Emoto M, Miyamoto M, Namba K, Schmits R, Van Rooijen N, Kita E, Kaufmann S H
Department of Immunology, Max-Planck-Institute for Infection Biology, Berlin, Germany.
Eur J Immunol. 2000 Oct;30(10):3049-56. doi: 10.1002/1521-4141(200010)30:10<3049::AID-IMMU3049>3.0.CO;2-F.
A distinct CD8+NKT cell population expressing TCRalpha/beta or TCRgamma/delta has been identified in liver and thymus. We wondered whether cell adhesion molecules play a role in the homing of CD8+NKT cells to the liver. The number of liver CD8+NKT cells was markedly reduced in leukocyte function-associated antigen (LFA)-1-/- mice compared with wild-type (WT) mice. The reduction was restricted to the liver only and no measurable alterations were found in other organs. In the liver of SCID mice reconstituted with thymocytes from LFA-1-/- or WT mice, the number of donor-derived CD8+NKT cells was comparable and the vast majority of these cells expressed TCRalpha/beta. In a reciprocal radiation thymocyte reconstitution system with LFA-1-/- and WT mice, LFA-1 expressed on liver cells other than CD8+NKT cells appeared to be required for the homing of thymic CD8+NKT cells to the liver. The accumulation of donor thymocyte-derived CD8+NKT cells in the liver of SCID mice was severely impaired by in vivo depletion of NK cells, but not of Kupffer cells. These results not only indicate that thymus provides a source for CD8+NKT cells expressing TCRalpha/beta in the liver, but also suggest that LFA-1 expressed on NK cells is involved in the homing of thymic CD8+NKT cells to the liver.
在肝脏和胸腺中已鉴定出一种表达TCRα/β或TCRγ/δ的独特CD8⁺NKT细胞群体。我们想知道细胞黏附分子是否在CD8⁺NKT细胞归巢至肝脏的过程中发挥作用。与野生型（WT）小鼠相比，白细胞功能相关抗原（LFA）-1⁻/⁻小鼠肝脏中的CD8⁺NKT细胞数量明显减少。这种减少仅局限于肝脏，在其他器官中未发现可测量的变化。在用LFA-1⁻/⁻或WT小鼠的胸腺细胞重建的SCID小鼠肝脏中，供体来源的CD8⁺NKT细胞数量相当，并且这些细胞中的绝大多数表达TCRα/β。在LFA-1⁻/⁻和WT小鼠的相互辐射胸腺细胞重建系统中，CD8⁺NKT细胞以外的肝细胞上表达的LFA-1似乎是胸腺CD8⁺NKT细胞归巢至肝脏所必需的。体内清除NK细胞可严重损害SCID小鼠肝脏中供体胸腺细胞来源的CD8⁺NKT细胞的积累，但清除库普弗细胞则不会。这些结果不仅表明胸腺为肝脏中表达TCRα/β的CD8⁺NKT细胞提供了来源，还表明NK细胞上表达的LFA-1参与了胸腺CD8⁺NKT细胞归巢至肝脏的过程。
