补体中间体C56与急性期人血清中酵母聚糖的结合。
Binding of the complement intermediate C56 to zymosan in acute phase human sera.
作者信息
Baker P J, Rubin L G, Lint T F, McLeod B C, Gewurz H
出版信息
Clin Exp Immunol. 1975 Apr;20(1):113-24.
Abstract
C56 is known to appear in the fluid phase when zymosan is incubated at 37 degrees C with certain acute phase 'reactor' sera. In the present study, C56 was detected bound to the zymosan particle prior to its appearance free in solution. In reactor sera C56 was formed and released with kinetics similar to that of the generation and decay of a C56-binding site formed when zymosan was incubated with normal serum. Bound and fluid phase C56 was detected only in reactor sera, and was generated only by agents known preferentially to activate the properdin pathway. Elution of C56 from zymosan in hypertonic salt solutions proved to be a convenient step in the partial purification of large amounts of this haemolytically active bimolecular complex.
摘要
已知当酵母聚糖在37℃与某些急性期“反应蛋白”血清一起孵育时，C56会出现在液相中。在本研究中，在溶液中游离的C56出现之前，就检测到C56与酵母聚糖颗粒结合。在反应蛋白血清中，C56的形成和释放动力学与酵母聚糖与正常血清孵育时形成的C56结合位点的产生和衰变相似。仅在反应蛋白血清中检测到结合的和液相的C56，并且仅由已知优先激活备解素途径的因子产生。在高渗盐溶液中从酵母聚糖上洗脱C56被证明是大量这种具有溶血活性的双分子复合物部分纯化中的一个方便步骤。
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本文引用的文献
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Immunochemistry. 1966 Mar;3(2):111-35. doi: 10.1016/0019-2791(66)90292-8.
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Lysis of erythrocytes by complement in the absence of antibody.在无抗体情况下补体对红细胞的溶解作用。
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Molecular analysis of the membrane attack mechanism of complement.补体膜攻击机制的分子分析
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The reaction of zymosan with the properdin system in normal and C4-deficienct guinea pig serum. Demonstration of C3- and C5-cleaving multi-unit enzymes, both containing factor B, and acceleration of their formation by the classical complement pathway.酵母聚糖与正常及C4缺陷豚鼠血清中备解素系统的反应。含B因子的C3和C5裂解多亚基酶的证实，以及经典补体途径对其形成的加速作用。
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Studies on the inhibition of C56-initiated lysis (reactive lysis). II. C567-INH--an inhibitor of the C567 trimolecular complex of complement.关于抑制C56起始裂解（反应性裂解）的研究。II. C567-INH——补体C567三分子复合物的一种抑制剂。
Int Arch Allergy Appl Immunol. 1974;47(4):623-32. doi: 10.1159/000231255.
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Reaction mechanisms of nascent C567 (reactive lysis). II. Killing of a rough form of Escherichia coli by C567, C8, and C9.新生C567的反应机制（反应性溶解）。II. C567、C8和C9对粗糙型大肠杆菌的杀伤作用。
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