Zendzian-Piotrowska M, Bucki R, Górska M, Górski J
Department of Physiology, Medical Academy of Bialystok, Poland.
Horm Metab Res. 2000 Oct;32(10):386-9. doi: 10.1055/s-2007-978659.
The aim of the present study was to examine the effect of acute streptozotocin diabetes on the content of different phospholipids and the incorporation of blood-borne 14C-palmitic acid into the phospholipid moieties in the rat liver nuclei. Diabetes was produced by intravenous administration of streptozotocin, and determinations were carried out two and seven days thereafter. Phospholipids were extracted from isolated nuclei and separated into the following fractions: sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylinositol, phosphatidylethanolamine and cardiolipin. Following that, they were quantified and radioactivity was measured. It was found that, in comparison to non-diabetic controls, two-day diabetes reduced the total content of phospholipids in the nuclei by 9.6%. The content of phospholipids in the nuclei by 9.6%. The content of phosphatidylcholine and phosphatidylserine was reduced and the content of the remaining phospholipids was stable. The specific activity of phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and cardiolipin, based on radioactivity incorporated from 14C-palmitic acid, was elevated. Seven-day diabetes resulted in a reduction of the total phospholipid content in the nuclei by 39.4%. This was accounted for by a reduction in the content of each phospholipid fraction with the exception of cardiolipin. The specific activity of each phospholipid fraction, was elevated in this group. It is concluded that insulin is involved in the regulation of the nuclear phospholipid content.
本研究的目的是检测急性链脲佐菌素诱导的糖尿病对大鼠肝细胞核中不同磷脂含量以及血源性14C-棕榈酸掺入磷脂部分的影响。通过静脉注射链脲佐菌素诱导糖尿病，此后两天和七天进行测定。从分离的细胞核中提取磷脂，并分离为以下组分：鞘磷脂、磷脂酰胆碱、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰乙醇胺和心磷脂。随后，对它们进行定量并测量放射性。结果发现，与非糖尿病对照组相比，糖尿病两天后细胞核中磷脂的总含量降低了9.6%。磷脂酰胆碱和磷脂酰丝氨酸的含量降低，其余磷脂的含量稳定。基于从14C-棕榈酸掺入的放射性，磷脂酰胆碱、磷脂酰丝氨酸、磷脂酰乙醇胺和心磷脂的比活性升高。糖尿病七天后，细胞核中总磷脂含量降低了39.4%。这是由于除心磷脂外，每个磷脂组分的含量均降低所致。该组中每个磷脂组分的比活性均升高。结论是胰岛素参与了核磷脂含量的调节。
