肾上腺髓质素与大鼠心肌收缩力
Adrenomedullin and myocardial contractility in the rat.
作者信息
Stangl V, Dschietzig T, Bramlage P, Boyé P, Kinkel H T, Staudt A, Baumann G, Felix S B, Stangl K
机构信息
Medizinische Klinik, Kardiologie, Angiologie, Pneumologie, Charité, Campus Mitte, Humboldt-Universität zu Berlin, Schumannstr. 20-21, D-10098 Berlin, Germany.
出版信息
Eur J Pharmacol. 2000 Nov 10;408(1):83-9. doi: 10.1016/s0014-2999(00)00765-2.
The effects of adrenomedullin in the regulation of myocardial contractility were investigated in the rat. In papillary muscles (n=6), adrenomedullin (0.1 to 10 nM) failed to show contractile effects. NO (nitric oxide) synthase inhibition with N(G)-nitro-L-arginine (L-NOARG) did not unmask any inotropic effect of adrenomedullin. The positive inotropic effect of isoprenaline (0. 01 nM to 10 microM) was identical after adrenomedullin, after L-NOARG, and after L-NOARG plus adrenomedullin (n=6 each). In field-stimulated rat ventricular myocytes, adrenomedullin (1, 10, and 100 nM; n=4 each) had impact neither on cell shortening nor on Ca(2+) transients. In isolated constant-flow perfused hearts (7.3+/-0.3 ml/min), adrenomedullin (1 nM, n=9; 10 nM, n=7) induced significant coronary vasodilation (-28%, -50%). In conclusion, adrenomedullin is a potent coronary vasodilator, but has no significant effects on myocardial contractility in the rat.
研究了肾上腺髓质素在大鼠心肌收缩调节中的作用。在乳头肌（n = 6）中，肾上腺髓质素（0.1至10 nM）未显示出收缩作用。用N(G)-硝基-L-精氨酸（L-NOARG）抑制一氧化氮（NO）合酶并未揭示肾上腺髓质素的任何变力作用。肾上腺素（0.01 nM至10 microM）的正性变力作用在给予肾上腺髓质素后、给予L-NOARG后以及给予L-NOARG加肾上腺髓质素后相同（每组n = 6）。在电场刺激的大鼠心室肌细胞中，肾上腺髓质素（1、10和100 nM；每组n = 4）对细胞缩短和Ca(2+)瞬变均无影响。在离体恒流灌注心脏（7.3±0.3 ml/min）中，肾上腺髓质素（1 nM，n = 9；10 nM，n = 7）引起显著的冠状动脉血管舒张（-28%，-50%）。总之，肾上腺髓质素是一种有效的冠状动脉血管舒张剂，但对大鼠心肌收缩力无显著影响。
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