McNamara J F, Lombardo H, Pillai S K, Jensen I, Albericio F, Kates S A
PerSeptive Biosystems Inc., Framingham, MA, USA.
J Pept Sci. 2000 Oct;6(10):512-8. doi: 10.1002/1099-1387(200010)6:10<512::AID-PSC269>3.0.CO;2-A.
Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/Bu(t) chemical approach and provide a general method to apply for the elongation of other difficult sequences.
通过逐步固相肽合成方法合成趋化因子已被证明是一项挑战。在此，结合配备反馈监测的连续流动合成器，对不同的偶联方法、溶剂和温度进行了全面研究。该研究结果表明，这类分子可以使用芴甲氧羰基/叔丁基（Fmoc/Bu(t)）化学方法制备，并提供了一种用于延伸其他困难序列的通用方法。
