Mohuczy D, Phillips M I
Department of Physiology, University of Florida, Gainesville, Florida 32610-0274, USA.
Methods. 2000 Nov;22(3):197-209. doi: 10.1006/meth.2000.1071.
Antisense inhibition is a method of attenuating the target at the gene expression level. There are two main groups of molecular tools for this goal. The first includes the use of short synthetic stretches of DNA-antisense oligodeoxynucleotides. The second tool is the use of vectors (plasmids or viruses) containing the gene of interest subcloned in the antisense orientation, which in the cells produces the antisense RNA. Both antisense DNA and RNA can bind to the complementary sense mRNA and interfere with its translation. Effects are usually short lasting (days) for oligodeoxynucleotides and longer lasting (weeks or months) for vectors. In this article we briefly describe techniques of antisense inhibition in the context of the renin-angiotensin system.
反义抑制是一种在基因表达水平上减弱靶标的方法。为此目的有两大类分子工具。第一类包括使用短的合成DNA片段——反义寡脱氧核苷酸。第二类工具是使用含有以反义方向亚克隆的目的基因的载体（质粒或病毒），其在细胞中产生反义RNA。反义DNA和RNA都可以与互补的有义mRNA结合并干扰其翻译。对于寡脱氧核苷酸，效应通常持续时间较短（数天），而对于载体则持续时间较长（数周或数月）。在本文中，我们在肾素-血管紧张素系统的背景下简要描述反义抑制技术。
